Article (Scientific journals)
Fast freezing of cow embryos in French straws with an automatic program.
Massip, A.; Van der Zwalmen, P.; Hanzen, Christian et al.
1982In Theriogenology, 18 (3), p. 325-332
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Abstract :
[en] Cow embryos between day 6.5 and 9 were frozen in 1.5M DMSO in PBS at 2 degrees C/min from seeding to -25 degrees C before being plunged into liquid nitrogen directly or after 10 min at -25 degrees C. Cooling rate from 20 degrees C to -5 degrees C was 9 degrees C/min. Seeding was induced automatically at -5 degrees C by injection of liquid nitrogen vapour. Embryos were subsequently thawed by direct transfer to water at 20 degrees C (group I) or at 37 degrees C (group II). Survival was assessed by culture in vitro and by transfer. In group I, 35.7% were degenerated after thawing (compared to 35.4% in group II). Survival rate after culture in vitro for 24h was not significantly different (48.3% vs 42.8%) and hatching rate after 96h culture was quite similar (33.3% vs 34.4%). In group II, four pregnancies were obtained from 10 embryos transferred. Time at -25 degrees C did not improve the results. Automatic seeding did not impair survival. These results show that the quality of the embryo is the determinant factor for survival after freezing and that the plastic straw is the most suitable vessel for freezing, storage and transfer of embryos.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Massip, A.
Van der Zwalmen, P.
Hanzen, Christian  ;  Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Obstétr. et path. de la reprod. des équidés, rumin. et porcs
Ectors, F.
Language :
English
Title :
Fast freezing of cow embryos in French straws with an automatic program.
Publication date :
1982
Journal title :
Theriogenology
ISSN :
0093-691X
eISSN :
1879-3231
Publisher :
Elsevier, Netherlands
Volume :
18
Issue :
3
Pages :
325-332
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 March 2009

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