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| Reference : Expression of growth hormone (GH)/insulin-like growth factor (IGF) axis during Balb/c on... |
| Scientific journals : Article | |||
| Human health sciences : Immunology & infectious disease Human health sciences : Endocrinology, metabolism & nutrition | |||
| http://hdl.handle.net/2268/89638 | |||
| Expression of growth hormone (GH)/insulin-like growth factor (IGF) axis during Balb/c ontogeny and effects of GH upon ex-vivo T-cell differentiation | |
| English | |
| Kermani, Hamid [Université de Liège - ULg > Centre d'Immunologie > > >] | |
| Goffinet, Lindsay [Université de Liège - ULg > > Centre d'immunologie >] | |
Mottet, Marie  [Université de Liège - ULg > > Centre d'immunologie >] | |
| Bodart, Gwennaëlle [Université de Liège - ULg > > > Doct. sc. bioméd. & pharma. (Bologne)] | |
| Morrhaye, Gabriel [Université de Liège - ULg > Centre d'Immunologie > > >] | |
| Dardenne, Olivier [Université de Liège - ULg > > Centre d'immunologie >] | |
| Overbergh, Lut [Katholieke Universiteit Leuven - KUL > Laboratory of Experimental Medicine and Endocrinology > > >] | |
| Baron, Frédéric [Université de Liège - ULg > > GIGA-R : Hématologie >] | |
| Beguin, Yves [Université de Liège - ULg > > GIGA-R : Hématologie >] | |
| Geenen, Vincent [Université de Liège - ULg > > Centre d'immunologie >] | |
| Martens, Henri [Université de Liège - ULg > > Centre d'immunologie >] | |
| 2012 | |
| Neuroimmunomodulation | |
| S. Karger | |
| 19 | |
| 137-147 | |
| International | |
| 1021-7401 | |
| 1423-0216 | |
| Basel | |
| Switzerland | |
| [en] Thymus ; Growth hormone receptor antagonist ; Insulin-like growth factors ; Fetal thymic organ cultures | |
| [en] Aims: We here address the question of expression and role of GH/IGF axis in the thymus.
Methods: Using RT-qPCR, the expression profile of various components of the somatotrope GH/IGF axis was measured in different thymic cell types and during thymus embryogenesis in Balb/c mice. Effect of GH on T-cell differentiation was explored through thymic organotypic culture. Results: Transcription of Gh, Igf1, Igf2 and their related receptors predominantly occurred in thymic epithelial cells (TEC), while a low level of Gh and Igf1r transcription was also evidenced in thymic T cells (thymocytes). Gh, Ghr, Ins2, Igf1, Igf2, and Igfr1, displayed distinct expression profiles depending on the developmental stage. The protein concentration of IGF-1 and IGF-2 were in accordance with the profile of their gene expression. In fetal thymus organ cultures (FTOC) derived from Balb/c mice, treatment with exogenous GH resulted in a significant increase of double negative CD4-CD8- T cells and CD4+ T cells, together with a decrease in double positive CD4+CD8+ T cells. These changes were inhibited by concomitant treatment with GH and GHR antagonist pegvisomant. However, GH treatment also induced a significant decrease in FTOC Gh, Ghr and Igf1 expression. Conclusion: These data show that the thymotropic properties of the somatotrope GH/IGF-1 axis involve an interaction between exogenous GH and GHR expressed by TEC. Since thymic IGF-1 is not increased by GH treatment, the effects of GH upon T-cell differentiation could implicate a different local growth factor or cytokine. | |
| Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE ; Pfizer Research Independent Grant ; Ministry of Health and Medical Education (Iran) | |
| Researchers ; Professionals | |
| http://hdl.handle.net/2268/89638 |
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