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Laminin binding and internalization by human and murine mammary gland cell lines in vitro.
Coopman, P. J.; Nuydens, R.; Leunissen, J. et al.
1991In European Journal of Cell Biology, 56 (2), p. 251-9
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Abstract :
[en] We have studied the binding and internalization of Engelbreth-Holm-Swarm mouse sarcoma laminin labeled with colloidal gold (LN-G40) by human and murine mammary gland cell lines. Interactions between the LN-G40 probe and the cells spread on a glass coverslip were monitored with video-enhanced contrast microscopy (Nanovid). Transmission electron microscopy allowed the quantitation of the LN-G40 probe at various cellular locations. During the first 15 min, a homogeneous binding of LN-G40 probe to the cell surface was observed with all cell lines. This binding did not occur with gold particles that were not conjugated to laminin. Then, the LN-G40 probe began to cluster on the cell surface and was, during the following 20 h, internalized by pits that were not coated. In the cells, the LN-G40 probe sometimes showed saltatory movements along linear tracks. The LN-G40 probe was intracellularly found in vesicles, multivesicular bodies, cisternal structures, and lysosomes, suggesting the degradation of the internalized laminin. However, not all cell surface-bound LN-G40 probe was internalized after 20 h. Differences between the cell lines were quantitative, but no clear correlation could be made between migration of cells on laminin and internalization of laminin.
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Coopman, P. J.
Nuydens, R.
Leunissen, J.
De Brabander, M.
Bortier, H.
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Mareel, M.
Language :
English
Title :
Laminin binding and internalization by human and murine mammary gland cell lines in vitro.
Publication date :
1991
Journal title :
European Journal of Cell Biology
ISSN :
0171-9335
eISSN :
1618-1298
Publisher :
Wissenschaftliche Verlagsgesellschaft, Stuttgart, Germany
Volume :
56
Issue :
2
Pages :
251-9
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 April 2011

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