ORBi: Lecler Renaud - (Functionnal) analysis of hydrogen production in Chlamydomonas reinhardtii mitochondrial mutants

Reference : (Functionnal) analysis of hydrogen production in Chlamydomonas reinhardtii mitochondrial...


	Document type :	Scientific congresses and symposiums : Poster
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/88983

Title :	(Functionnal) analysis of hydrogen production in Chlamydomonas reinhardtii mitochondrial mutants
Language :	English
Alternative title :	[fr] Analyse fonctionnelle de la production d'hydrogène chez des mutants mitochondrieux de Chlamydomonas reinhardtii
Author, co-author :	Lecler, Renaud [Université de Liège - ULg > > Labo de Bioénergétique >]
	Godaux, Damien [Université de Liège - ULg > Département des sciences de la vie > Génétique >]
	Hamilton, Christopher [Université de Liège - ULg > > Centre Wallon de biologie industrielle >]
	Hiligsmann, Serge [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
	Franck, Fabrice [Université de Liège - ULg > > Labo de Bioénergétique >]
	Remacle, Claire [Université de Liège - ULg > Département des sciences de la vie > Génétique >]
Publication date :	27-Jun-2010
Page number :	A5
Peer reviewed :	No
On invitation :	No
Audience :	International
Event name :	9th International Hydrogenase conference
Event date :	du 27 juin 2010 au 02 juillet 2010
Event organizer :	AKADEMIKONFERENS (UPPSALA UNIVERSITET)
Event place (city) :	Uppsala
Event country :	Sweden
Keywords :	[en] Chlamydomonas ; Hydrogen ; Mitochondria
Abstract :	[en] Mitochondrial Chlamydomonas mutants for respiratory complexes present a decreased dark respiration and apparent yield of photosynthetic linear electron flow. They accumulate reducing power such as NAD(P)H and show lower levels of ATP. Under restrictive conditions, like sulfur depletion and anoxia, Chlamydomonas is able to produce hydrogen towards the activation of a chloroplatic O2-sensitive Fe-hydrogenase which catalyses the reduction of electrons to H2. In this study we used an adapted Melis protocol to analyse hydrogen evolution of mitochondrial mutants. For this aim a simple-flask system was built with gaz collecting tubes. A parallel flask was used for GC analyses.
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/88983

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