Reference : Support fermé: une réalité clinique pour vitrifier en conditions aseptiques les ovocytes...
Scientific journals : Article
Human health sciences : Reproductive medicine (gynecology, andrology, obstetrics)
http://hdl.handle.net/2268/88912
Support fermé: une réalité clinique pour vitrifier en conditions aseptiques les ovocytes et embryons
French
[en] Closed carrier device: A reality to vitrify oocytes and embryos in aseptic conditions
Vanderzwalmen, Pierre mailto [Université de Liège - ULg > > > Doct. sc. vété. (Bologne)]
Zech, Nicolas [ > > ]
Prapas, Y. [ > > ]
Panagiotidis, Y. [ > > ]
Papatheodorou, A. [ > > ]
Lejeune, Bernard [ > > ]
Jareno, D. [ > > ]
Vanderzwalmen, Sabine [ > > ]
Ectors, Fabien mailto [Université de Liège - ULg > > GIGA-Management : Plate-forme transgénique >]
2010
Gynécologie Obstétrique & Fertilité
Elsevier Science
38
9
541-546
Yes (verified by ORBi)
International
1297-9589
[en] human embryo ; Cryopreservation/methods ; vitrification
[en] Vitrification with the use of ‘‘Open’’ carrier devices (Cryoloop, cryotop, cryoleaf, Vitriplug) which allowed
the contact with liquid nitrogen has become a more popular way to achieve cooling rate superior to
20.000 °C/min. Even though the question of contamination with liquid nitrogen during ultra-rapid
cooling and storage remain debatable with the use of ‘‘open’’ devices, it is important to revise the carrier system in a way, which minimizes the risk of contamination. According to the EU tissues and cells directive, it is advisable that the cooling and storage should be carried out in embryo carrier devices ensuring complete separation of the embryos from liquid nitrogen in a way, which minimizes the risk of contamination. The consequence of a reduction in the cooling rate resulting from the heat-insulating barrier of aseptic devices has to be counteracted by gradually increasing intracellular concentrations of cryoprotectants without inducing a toxic effect. We developed an aseptic vitrification method of vitrification for MII oocytes and embryos at different stage of development using the ‘‘VitriSafe’’ as ‘‘closed’’ carrier device.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/88912

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