Reference : La vitrification et l’utilisation de concentrations élevées en cryoprotecteurs : ceci...
Scientific journals : Article
Human health sciences : Reproductive medicine (gynecology, andrology, obstetrics)
La vitrification et l’utilisation de concentrations élevées en cryoprotecteurs : ceci justifie-t-il la préférence pour la congélation lente ?
[en] Vitrification and the use of high concentrations of cryoprotectants: Is it a justified argument to prefer slow freezing?
Vanderzwalmen, Pierre mailto [Université de Liège - ULg > > > Doct. sc. vété. (Bologne)]
Zech, Nicolas [> >]
Lejeune, Bernard [> >]
Wirtleitner, Barbara [> >]
Zech, Nicolas [> >]
Ectors, Fabien mailto [Université de Liège - ULg > > GIGA-Management : Plate-forme transgénique >]
Gynécologie Obstétrique & Fertilité
Elsevier Science
Yes (verified by ORBi)
[en] Etat vitrifiant ; Cryoprotecteurs ; vitrification
[en] The use of high levels of cryoprotectants (CPs) in solutions applied to vitrify oocytes or embryos is an argument to still prefer slow freezing procedure. Is it a justified argument? Out of three studies using mice zygotes we may assume that (i) the intracellular concentration of CPs is far lower than the one in the vitrification solutions, (ii) the intracellular concentration of CPs in the vitrified zygote is in contrary to the common beliefs even lower than the one observed after a slow freezing procedure, (iii) survival after slow freezing reflects the presence of an intracellular vitrified state in these cells.
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