Article (Scientific journals)
Development and validation of stable reference materials for food microbiology using Bacillus cereus and Clostridium perfringens spores
Abdelmassih, Marleen; Planchon, Viviane; Anceau, Christine et al.
2011In Journal of Applied Microbiology
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Keywords :
Bacillus cereus; Clostridium perfringens; food microbiology; reference material; spore; validation
Abstract :
[en] To develop a new type of microbiological Reference Materials (RMs), displaying long-term stability at room temperature. The purpose was to produce and validate two batches of RMs for the enumeration of Bacillus cereus and Clostridium perfringens. Methods and Results: The RMs were based on spores of B. cereus and Cl. perfringens, adsorbed on calcium carbonate pellets. Two batches of 1000 units were manufactured and validated in compliance with ISO guide 35. After verification of their homogeneity, the stability of the ‘RM-B. cereus’ and ‘RMCl. perfringens’ batches was proven during at least 36 and 9 months, respectively, at room temperature. The validation study was completed by international collaborative trial involving 12 laboratories, allowing the validation of the assigned values. Conclusions: The methodology developed in this work enabled to produce easy-to-handle and cost-effective RMs, displaying an unprecedented stability at room temperature, a good homogeneity and a precise and validated assigned value. Significance and Impact of the Study: This study revealed new paths for the development of stable microbiological RMs. Overcoming the intrinsic instability of the living cells makes it possible to produce valuable tools for the quality assurance of microbiology laboratories. Jou
Disciplines :
Microbiology
Author, co-author :
Abdelmassih, Marleen
Planchon, Viviane
Anceau, Christine ;  Université de Liège - ULiège > Chimie et bio-industries > Technologie des industries agro-alimentaires
Mahillon, Jacques
Language :
English
Title :
Development and validation of stable reference materials for food microbiology using Bacillus cereus and Clostridium perfringens spores
Publication date :
12 April 2011
Journal title :
Journal of Applied Microbiology
ISSN :
1364-5072
eISSN :
1365-2672
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Convention cadre REQUASUD
Funders :
Service Public de Wallonie - DGO3
Available on ORBi :
since 14 April 2011

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