|Reference : Evaluation of the long-term barrier effect of commercial resorbable guided tissue regene...|
|Scientific congresses and symposiums : Poster|
|Human health sciences : Dentistry & oral medicine|
|Evaluation of the long-term barrier effect of commercial resorbable guided tissue regenerative membranes : an in vitro study using human gingival fibroblasts|
|Grenade, Charlotte [Université de Liège - ULg > > Institut de dentisterie - prothèse fixée >]|
|Borget, Pascal [> >]|
|Moniotte, Nicolas [> >]|
|Pevee, Jean-Michel [Université de Liège - ULg > > Institut de dentisterie - prothèse fixée >]|
|Humblet, Chantal [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]|
|Rompen, Eric [Université de Liège - ULg > Département de science dentaire > Chirurgie bucco-dentaire et parodontologie >]|
|Gillet, Marie-Claire [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]|
The first part of the study devoted to guided tissue regenerative membranes was focused on a better understanding of the physicochemical and mechanical properties of commercial materials.
The second objective of our study was to develop an in vitro device able to measure the long-term barrier effect of resorbable membranes.
After the development of this new device, experiments were realized to characterize the long-term behaviour of commercially membranes with human gingival fibroblasts (HGF).
Materials and methods
The use of human gingival fibroblastic cells was chosen to get closer to biological conditions. Some gingival explants were removed in young and non-smoking healthy patients. From these explants, fibroblastic cells were isolated and cultivated. These cells will be able to be used between the third and the sixth passage.
Resorbable membranes were chosen because they don’t require a second surgical operation. There are made of polyesters or collagen.
A system based on inserts was developed in order to follow the degradation of membranes and the migration of cells across the material. The membrane was cut into 8 mm diameter punches and set in the bottom of the system. Once the whole was put together, it is laced into a 12 wells plate culture.
First, the plates were put in an incubator at 37°C, during times ranging from 24 hours to several months. The barrier effect was then measured to reflect the gradual increase of permeability of each membrane.
For this purpose, HGF were seeded on the different samples. The top of the bottle and the bottom of the well were then filled with culture medium.
Non degradable synthetic Bioflex membranes were chosen as control samples which don't let pass cells (porosity : 0,4 µm).
After 48 hours of incubation in the presence of cells, pictures of cells on membranes and in the bottom of wells were taken with an optic microscope. Viability tests (MTS) were then realized on membranes to evaluate cells proliferation and in the bottom of wells to measure barrier effect.
Finally, the morphology of cells on selected membranes was characterized by Scanning Electron Microscopy.
Proliferation results correspond to data published by several authors. Furthermore, the barrier effect times found in the present study are similar to barrier effect times demonstrated in in vivo studies and announced by manufacturers.
In conclusion, the finalized system is adapted to the analysis of long-term barrier effect of commercial GTR membranes. This system will be tested with synthetic bioresorbable membranes made of copolymers.
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