Reference : Plasma urea nitrogen in relation to pregnancy rate in dairy sheep
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/88735
Plasma urea nitrogen in relation to pregnancy rate in dairy sheep
English
Karen, Aly [Kafrelsheikh University, Egypt > Department of Theriogenology, Faculty of Veterinary Medicine > > >]
Beckers, Jean-François mailto [> > > >]
Melo de Sousa, Noelita mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Szenci, Otto [Szent István University, H-2225 Üllo-Dóra Major, Hungary > Clinic for Large Animals, Faculty of Veterinary Science > > >]
2011
Animal reproduction science
124
1-2
69-72
Yes (verified by ORBi)
International
0378-4320
1873-2232
Netherlands
[en] Plasma urea nitrogen ; Pregnancy rate ; Sheep
[en] The aim of this field study was to investigate the relationship of plasma urea nitrogen (PUN) with the pregnancy rate in lactating AwassixMerino ewes. One hundred and eighty-five AwassixMerino ewes were used in the present study. Ewes were fed a diet containing 17.4% crude protein and were milked twice a day by the milking machine. The ewes were synchronized for estrus by insertion of intravaginal sponges containing 30mg flurogestone acetate for 14 days. At the time of sponge removal each ewe was administered eCG (600IU). All ewes were inseminated twice with fresh semen into the external os of the cervix at 48 and 56h after sponge removal. The day of insemination was considered as Day 0 for calculating the gestational period. Blood samples were collected from each ewe at Days 0, 18 for measurement of PUN concentrations and at Day 22 after AI for measurement of pregnancy-associated glycoprotein (PAG) by radioimmunoassay (RIA). Thirty-eight ewes (20.5%) were confirmed pregnant by PAG-RIA test at Day 22 and by ultrasonography at Day 80. The mean (+/-S.D.) concentration of PUN in all ewes at Day 0 was 12.7+/-4.6mmol/L. There were non-significant differences in the level of PUN between pregnant and non-pregnant ewes at Days 0 (12.2+/-4.2mmol/L vs. 12.8+/-4.7mmol/L, respectively) and 18 (9.6+/-2.9mmol/L vs. 10.4+/-4.0mmol/L, respectively) after AI. Mean PUN concentrations decreased significantly from Day 0 to Day 18 after AI in both pregnant and non-pregnant ewes. By using logistic regression analysis, there was no effect of PUN concentrations on the probability of pregnancy occurrence in the studied ewes (odds ratio: 0.97; 95% confidence interval: 0.9-1.05; P=0.45). In conclusion, there was no evidence of a relationship between PUN concentration and pregnancy rate for lactating AwassixMerino ewes in the present study because of low pregnancy rate observed.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/88735
10.1016/j.anireprosci.2011.01.012

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