Article (Scientific journals)
SHIP2 controls PtdIns(3,4,5)P3 and PKB activity in response to oxidative stress
Zhang, J.; Liu, Z.; Rasschaert, J. et al.
2007In Cellular Signalling, 19, p. 2194-2200
Peer Reviewed verified by ORBi
 

Files


Full Text
Zhang_2007_Cellular-Signalling.pdf
Publisher postprint (765.09 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] Reactive oxygen species (ROS) are known to be involved in redox signalling pathways that may contribute to normal cell function as well as disease progression. The tumour suppressor PTEN and the inositol 5-phosphatase SHIP2 are critical enzymes in the control of PtdIns(3,4,5)P(3) level. It has been reported that oxidants, including those produced in cells such as macrophages, can activate downstream signalling via the inactivation of PTEN. The present study evaluates the potential impact of SHIP2 on phosphoinositides in cells exposed to sodium peroxide. We used a model of SHIP2 deficient mouse embryonic fibroblasts (MEFs) stimulated by H(2)O(2): at 15 min, PtdIns(3,4,5)P(3) was markedly increased in SHIP2 -/- cells as compared to +/+ cells. In contrast, no significant increase in PtdIns(3,4)P(2) could be detected at 15 or 120 min incubation of the cells with H(2)O(2) (0.6 mM). PKB activity was also upregulated in SHIP2 -/- cells as compared to +/+ cells in response to H(2)O(2). SHIP2 add back experiments in SHIP2 -/- cells confirm its critical role as a lipid phosphatase in the control of PtdIns(3,4,5)P(3) level in response to H(2)O(2). We conclude that SHIP2 lipid phosphatase activity plays an important role in the metabolism PtdIns(3,4,5)P(3) which is demonstrated in oxygen stressed cells
Disciplines :
Biochemistry, biophysics & molecular biology
Genetics & genetic processes
Author, co-author :
Zhang, J.;  Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire (IRIBHM)
Liu, Z.;  Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire (IRIBHM)
Rasschaert, J.;  Université Libre de Bruxelles - ULB > Laboratory of Experimental Medicine, Faculty of Medicine
Blero, D.;  Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire (IRIBHM)
Deneubourg, L.;  Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire (IRIBHM)
Schurmans, Stéphane  ;  Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire en Biologie Humaine et Moleculaire
Erneux, C.;  Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire (IRIBHM)
Pesesse, X.;  Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire (IRIBHM)
Language :
English
Title :
SHIP2 controls PtdIns(3,4,5)P3 and PKB activity in response to oxidative stress
Publication date :
2007
Journal title :
Cellular Signalling
ISSN :
0898-6568
eISSN :
1873-3913
Publisher :
Elsevier Science, Oxford, United Kingdom
Volume :
19
Pages :
2194-2200
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 January 2010

Statistics


Number of views
31 (1 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
21
Scopus citations®
without self-citations
13
OpenCitations
 
22

Bibliography


Similar publications



Contact ORBi