ORBi: Van Der Heiden Edwige - Food flavonoid aryl hydrocarbon receptor-mediated agonistic/antagonistic/synergic activities in human and rat reporter gene assays

Reference : Food flavonoid aryl hydrocarbon receptor-mediated agonistic/antagonistic/synergic activi...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/8797

Title :	Food flavonoid aryl hydrocarbon receptor-mediated agonistic/antagonistic/synergic activities in human and rat reporter gene assays
Language :	English
Author, co-author :	Van Der Heiden, Edwige [Université de Liège - ULg > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires >]
	Bechoux, Nathalie [Université de Liège - ULg > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires >]
	Muller, Marc [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
	Sergent, Thérèse [ > > ]
	Schneider, Yves-Jacques [ > > ]
	Larondelle, Yvan [ > > ]
	Maghuin-Rogister, Guy [Université de Liège - ULg > Département de sciences des denrées alimentaires > Département de sciences des denrées alimentaires >]
	Scippo, Marie-Louise [Université de Liège - ULg > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires >]
Publication date :	2009
Journal title :	Analytica Chimica Acta
Publisher :	Elsevier
Volume :	637
Pages :	337-345
Audience :	International
ISSN :	0003-2670
e-ISSN :	1873-4324
City :	Amsterdam
Country :	The Netherlands
Abstract :	[en] Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor mediating the adverse effects of dioxins and polycyclic aromatic hydrocarbons (PAHs). In this study, we investigated the genetic-, time-, dose-, species- and tissue-dependent AhR-mediated agonistic/ antagonistic activities of three food flavonoids: quercetin, chrysin and genistein. To that end, four stably transfected cell lines were used in cell-based luciferase reporter gene assays: three lines were transformed with the ptKLuc vector harbouring four dioxinresponsive elements (DREs) upstream of the thymidine kinase promoter and the luciferase gene (HepG2-Luc, T-47D-Luc and H4IIE-ULg). The fourth is a patented cell line transformed with a different construct: H4IIE DR-CALUX®. Both H4IIE cells were compared for their genetic construction. Human hepatoma (HepG2-Luc) and human breast tumour (T-47D-Luc) cells were compared for tissue-dependent effects. Rat hepatoma (H4IIE-ULg) and human hepatoma (HepG2-Luc) cellswere compared for species-dependent activities.We concluded that quercetin, chrysin and genistein act in a time-, dose-, species- and tissue-specific way. For example, genistein displayed agonistic activities when exposed to rat hepatoma cells during 6h but not after 24 h. Flavonoids displayed agonistic/antagonistic activities in human breast tumour cells, depending on the exposure time, while in human hepatoma cells, only antagonistic activities of flavonoids were measured. In addition, we report, in all the cells, a synergy between an isoflavone and two food contaminants; the 2,3,7,8-tetrachlorodibenzop- dioxin and 3-methylcholanthrene, a PAH. In rat cells, this synergy occurred when cells were exposed to flavonoids and contaminant for 6h, while it was observed in human cells only after 24 h.
Permalink :	http://hdl.handle.net/2268/8797

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