Reference : Comparative study of Ag and Au nanoparticles biosensors based on surface plasmon reso...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Physics
http://hdl.handle.net/2268/87949
Comparative study of Ag and Au nanoparticles biosensors based on surface plasmon resonance phenomenon
English
Lismont, Marjorie mailto [Université de Liège - ULg > Département de physique > Biophotonique >]
Dreesen, Laurent mailto [Université de Liège - ULg > Département de physique > Biophotonique >]
2012
Materials Science & Engineering : C
Elsevier Science
Yes (verified by ORBi)
International
0928-4931
Amsterdam
The Netherlands
[en] Biosensors ; Nanoparticles
[en] The specific sensitivity of surface plasmon resonance to changes in the local environment of nanoparticles allows their use as platforms to probe chemical and biochemical binding events on their surfaces without any labeling [1- 4]. In this paper, we perform a comparative study of gold and silver nanoparticle based biosensors, prepared within the same conditions, in order to determine which metal seems the best for biological sensing. The prototypical biocytin-avidin
interaction is used to study gradual changes over time and with avidin concentration in the absorption spectra bands of biocytinylated 10 nm silver and gold nanospheres. First, the Ag nanoparticles plasmon resonance absorbance signal is about 10 times larger than the Au one. Secondly, for an equivalent concentration of avidin, the optical properties modifications are more pronounced for silver nanoparticles than for gold ones of the same geometry. These observations
attest the superiority of Ag on Au nanoparticles when optical considerations are only taken into account. Finally, with both biosensors, the specificity of the interaction, checked by replacing avidin with bovine serum albumin, is relatively poor and needs to be improved.
Researchers
http://hdl.handle.net/2268/87949

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Ag and Au NP biosensors.pdfAuthor preprint726.7 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.