Reference : Alternative attachment factors and internalization pathways for GIII.2 bovine noroviruses.
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/87791
Alternative attachment factors and internalization pathways for GIII.2 bovine noroviruses.
English
Mauroy, Axel mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie vétérinaire et maladies virales animales >]
Gillet, Laurent mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Mathijs, Elisabeth mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie vétérinaire et maladies virales animales >]
Vanderplasschen, Alain mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Thiry, Etienne mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie vétérinaire et maladies virales animales >]
2011
Journal of General Virology (The)
Society for General Microbiology
Yes (verified by ORBi)
International
0022-1317
1465-2099
London
United Kingdom
[en] Bovine noroviruses belong to the family Caliciviridae, genus Norovirus. Two genotypes are described and viruses genetically related to the Jena and Newbury-2 strains are classified into genotypes 1 and 2 respectively. In this study, virus-like particles (VLP) of the previously detected B309 Belgian strain, genetically related to genotype 2 bovine noroviruses, were used to investigate virus-host interactions in vitro. B309 VLP were shown to bind to several bovine cell lines. This binding was not affected by heparinase or chondroitinase treatment but was significantly inhibited by both sodium periodate, alpha-galactosidase, trypsin and phospholipase C treatment. Cell treatment by neuraminidase also moderately affected this binding. Taken together, these results show that, in addition to a galactosyl residue, sialic acid could also be involved in binding to susceptible cells. In addition, both the cholesterol-dependent pathway and macropinocytosis are used for B309 VLP internalisation by Madin-Darby Bovine Kidney cells. The data increase the knowledge on bovine norovirus cell interactions.
http://hdl.handle.net/2268/87791
10.1099/vir.0.030072-0

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