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Adenohypophysis hormone gene products in 14 pituitary adenomas: analysis by immunohistochemistry and northern blotting.
Scippo, Marie-Louise; Beckers, Albert; Frankenne, Francis et al.
1991In Archives Internationales de Physiologie, de Biochimie et de Biophysique, 99 (2), p. 135-40
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Keywords :
Adenoma/chemistry/genetics; Blotting, Northern; Gene Expression; Humans; Immunohistochemistry; Oligonucleotides/biosynthesis; Pituitary Hormones, Anterior/analysis/genetics; Pituitary Neoplasms/chemistry/genetics; RNA, Messenger/biosynthesis
Abstract :
[en] We investigated 14 pituitary adenomas (10 silent adenomas; 3 prolactinomas and one GH-secreting tumor) for the presence of hormone gene transcripts (Northern blot) as well as for translation products (immunohistochemistry). The GH-secreting tumor was shown to express the genes coding for GH and PRL and to synthesize the corresponding hormones. In the cases of prolactinomas, immunohistochemical data demonstrated the synthesis of prolactin only. In addition to the PRL gene, Northern blot analysis revealed the transcription of the alpha-subunit gene in one case. Hormone genes were found to be expressed in 7 out of the 10 silent tumors, whereas no hormone synthesis was detected in any of these tissues. LH-beta mRNA was found in 3 cases, FSH-beta mRNA in 5 cases and alpha-subunit gene was shown to be expressed in one case. Surprisingly, the level of expression of the FSH-beta gene was higher than in normal tissue. This study confirms that some so called "silent" adenomas are expressing alpha- and/or beta-subunit glycoprotein hormone genes, even if no hormone is synthesized. The therapeutic action of bromocriptine described in some "silent" adenomas cases could be related to that hormone gene expression potentiality.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Scippo, Marie-Louise  ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Beckers, Albert ;  Université de Liège - ULiège > Département des sciences cliniques > Endocrinologie
Frankenne, Francis ;  Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
Reznik, Michel ;  Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Stevenaert, Achille ;  Centre Hospitalier Universitaire de Liège - CHU > Neurochirurgie
Igout, Ahmed ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Hennen, Georges ;  Centre Hospitalier Universitaire de Liège - CHU > Endocrinologie clinique
Language :
English
Title :
Adenohypophysis hormone gene products in 14 pituitary adenomas: analysis by immunohistochemistry and northern blotting.
Publication date :
1991
Journal title :
Archives Internationales de Physiologie, de Biochimie et de Biophysique
ISSN :
0778-3124
Publisher :
Vaillant Carmanne, Liège, Belgium
Volume :
99
Issue :
2
Pages :
135-40
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 13 March 2009

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