Reference : Adenohypophysis hormone gene products in 14 pituitary adenomas: analysis by immunohistoc...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Adenohypophysis hormone gene products in 14 pituitary adenomas: analysis by immunohistochemistry and northern blotting.
Scippo, Marie-Louise mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires >]
Beckers, Albert mailto [Université de Liège - ULg > Département des sciences cliniques > Endocrinologie >]
Frankenne, Francis [Université de Liège - ULg > Département des sciences cliniques > Département des sciences cliniques >]
Reznik, Michel [Université de Liège - ULg > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]
Stevenaert, Achille [Université de Liège - ULg > > Neurochirurgie >]
Igout, Ahmed [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Hennen, Georges [Université de Liège - ULg > > Endocrinologie clinique >]
Archives Internationales de Physiologie, de Biochimie et de Biophysique
Vaillant Carmanne
Yes (verified by ORBi)
[en] Adenoma/chemistry/genetics ; Blotting, Northern ; Gene Expression ; Humans ; Immunohistochemistry ; Oligonucleotides/biosynthesis ; Pituitary Hormones, Anterior/analysis/genetics ; Pituitary Neoplasms/chemistry/genetics ; RNA, Messenger/biosynthesis
[en] We investigated 14 pituitary adenomas (10 silent adenomas; 3 prolactinomas and one GH-secreting tumor) for the presence of hormone gene transcripts (Northern blot) as well as for translation products (immunohistochemistry). The GH-secreting tumor was shown to express the genes coding for GH and PRL and to synthesize the corresponding hormones. In the cases of prolactinomas, immunohistochemical data demonstrated the synthesis of prolactin only. In addition to the PRL gene, Northern blot analysis revealed the transcription of the alpha-subunit gene in one case. Hormone genes were found to be expressed in 7 out of the 10 silent tumors, whereas no hormone synthesis was detected in any of these tissues. LH-beta mRNA was found in 3 cases, FSH-beta mRNA in 5 cases and alpha-subunit gene was shown to be expressed in one case. Surprisingly, the level of expression of the FSH-beta-gene was higher than in normal tissue. This study confirms that some so called << silent >> adenomas are expressing alpha- and/or beta-subunit glycoprotein hormone genes, even if no hormone is synthesized. The therapeutic action of bromocriptine described in some << silent >> adenomas cases could be related to that hormone gene expression potentiality.
Researchers ; Professionals

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