Reference : Adenohypophysis hormone gene products in 14 pituitary adenomas: analysis by immunohistoc...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/8719
Adenohypophysis hormone gene products in 14 pituitary adenomas: analysis by immunohistochemistry and northern blotting.
English
Scippo, Marie-Louise mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires >]
Beckers, Albert mailto [Université de Liège - ULg > Département des sciences cliniques > Endocrinologie >]
Frankenne, Francis [Université de Liège - ULg > Département des sciences cliniques > Département des sciences cliniques >]
Reznik, Michel mailto [Université de Liège - ULg > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]
Stevenaert, Achille [Centre Hospitalier Universitaire de Liège - CHU > > Neurochirurgie >]
Igout, Ahmed mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Hennen, Georges [Centre Hospitalier Universitaire de Liège - CHU > > Endocrinologie clinique >]
1991
Archives Internationales de Physiologie, de Biochimie et de Biophysique
Vaillant Carmanne
99
2
135-40
Yes (verified by ORBi)
International
0778-3124
Liège
Belgique
[en] Adenoma/chemistry/genetics ; Blotting, Northern ; Gene Expression ; Humans ; Immunohistochemistry ; Oligonucleotides/biosynthesis ; Pituitary Hormones, Anterior/analysis/genetics ; Pituitary Neoplasms/chemistry/genetics ; RNA, Messenger/biosynthesis
[en] We investigated 14 pituitary adenomas (10 silent adenomas; 3 prolactinomas and one GH-secreting tumor) for the presence of hormone gene transcripts (Northern blot) as well as for translation products (immunohistochemistry). The GH-secreting tumor was shown to express the genes coding for GH and PRL and to synthesize the corresponding hormones. In the cases of prolactinomas, immunohistochemical data demonstrated the synthesis of prolactin only. In addition to the PRL gene, Northern blot analysis revealed the transcription of the alpha-subunit gene in one case. Hormone genes were found to be expressed in 7 out of the 10 silent tumors, whereas no hormone synthesis was detected in any of these tissues. LH-beta mRNA was found in 3 cases, FSH-beta mRNA in 5 cases and alpha-subunit gene was shown to be expressed in one case. Surprisingly, the level of expression of the FSH-beta gene was higher than in normal tissue. This study confirms that some so called "silent" adenomas are expressing alpha- and/or beta-subunit glycoprotein hormone genes, even if no hormone is synthesized. The therapeutic action of bromocriptine described in some "silent" adenomas cases could be related to that hormone gene expression potentiality.
http://hdl.handle.net/2268/8719

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