[en] The hGH/hCS genes, clustered on chromosome 17 in the 5' to 3' order GH-N, CS-L, CS-A, GH-V and CS-B, show a high degree of sequence identity. The expression product of the GH-V gene is the placental growth hormone, which replaces pituitary GH in maternal blood throughout pregnancy. By means of mRNA competitive hybridization using P-32-labelled and unlabelled 30 bases long oligonucleotides, we first optimized specific hybridization conditions. In situ hybridization was then performed to locate the GH-V mRNA encoding placental growth hormone. The hGH-V gene appears expressed in the placental syncytiotrophoblast. Unlike the CS-A and CS-B genes (both encoding hPL) which are expressed uniformly in the syncytiotrophoblast, the GH-V mRNA is located in a few syncytiotrophoblast cells only.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Scippo, Marie-Louise ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Frankenne, Francis ; Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
HOOGHEPETERS, ELisabeth
Igout, Ahmed ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
VELKENIERS, Brigitte
Hennen, Georges ; Centre Hospitalier Universitaire de Liège - CHU > Endocrinologie clinique
Language :
English
Title :
SYNCYTIOTROPHOBLASTIC LOCALIZATION OF THE HUMAN GROWTH-HORMONE VARIANT MESSENGER-RNA IN THE PLACENTA
Publication date :
1993
Journal title :
Molecular and Cellular Endocrinology
ISSN :
0303-7207
eISSN :
1872-8057
Publisher :
North Holland Publishing Company, Amsterdam, Netherlands
Liebhaber, Urbanek, Ray, Tuan, Cooke J. Clin. Invest. 1989, 83:1985-1991.
Marcotty, Frankenne, Scippo (1992) 74th Annual Meeting of the Endocrine Society. 74th Annual Meeting of the Endocrine Society, Program and Abstracts, abstract No. 1424.; .
Pelligrini, Lebrun, Ali, Kelly Mol. Endocrinol. 1992, 6:1023-1031.
Ray, Jones, Liebhaber, Cooke J. Clin. Endocrinol. Metab. 1989, 125:566-568.
Seeburg DNA 1982, 1:239-249.
Thomas Proc. Natl. Acad. Sci. USA 1980, 205:602-606.