radio-receptor assay; steroid hormone receptor; veterinary drug residue; anabolics
Abstract :
[en] In the European Union (EU), the use of growth-promoting substances in meat production is banned. The control of growth promoters, especially steroid hormones, is presently based on expensive and time-consuming chromatographic methods of analysis or, sometimes, for screening purposes, on radio- or enzyme-immunoassays, all of which are often too specific to allow effective multi-analyte control. In order to develop rapid and inexpensive multi-analyte detection tests, we proposed the use of hormonal receptors as detection tools. The system described here (radio-receptor assays) is based on a direct bindin g assay of steroid hormones to their respective receptors. Human receptors to estrogens (hERalpha), androgens (hAR), progestagens (hPR) and glucocorticoids (hGR) have been produced by genetic engineering in bacteria or in eucaryotic cells. Binding analyses revealed that the obtained receptor proteins retained a high affinity for their corresponding native ligand. In addition, competition studies continued that each of the four receptors displays a specificity profile for a series of analogs in agreement with the literature. Finally, the stability of these recombinant receptors is sufficient to allow their use in test kits. (C) 2002 Elsevier Science B.V. All rights reserved.
Disciplines :
Chemistry
Author, co-author :
Scippo, Marie-Louise ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Van de Weerdt, Cécile ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Willemsen, Philippe
François, Jean-Marie ; Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Rentier-Delrue, Françoise ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Muller, Marc ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Maghuin-Rogister, Guy ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Département de sciences des denrées alimentaires
Language :
English
Title :
Detection of illegal growth promoters in biological samples using receptor binding assays
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