ORBi: Willemsen Philippe - Use of specific bioluminescent cell lines for the detection of steroid hormone (ant)agonists in meat producing animals

Reference : Use of specific bioluminescent cell lines for the detection of steroid hormone (ant)agon...


	Document type :	Scientific journals : Article
	Discipline(s) :	Physical, chemical, mathematical & earth Sciences : Chemistry
	To cite this reference:	http://hdl.handle.net/2268/8708

Title :	Use of specific bioluminescent cell lines for the detection of steroid hormone (ant)agonists in meat producing animals
Language :	English
Author, co-author :	Willemsen, Philippe [> > > >]
	Scippo, Marie-Louise [Université de Liège - ULg > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires >]
	Maghuin-Rogister, Guy [Université de Liège - ULg > Département de sciences des denrées alimentaires > Département de sciences des denrées alimentaires >]
	Martial, Joseph [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
	Muller, Marc [Université de Liège - ULg > Département des sciences de la vie > Biologie et génétique moléculaire >]
Publication date :	2002
Journal title :	Analytica Chimica Acta
Publisher :	Elsevier Science Bv
Volume :	473
Issue/season :	1-2
Pages :	119-126
Audience :	International
ISSN :	0003-2670
e-ISSN :	1873-4324
City :	Amsterdam
Keywords :	[en] bioluminescence ; luciferase reporter gene ; steroid hormones ; transcription ; food ; residues ; progesterone ; dexamethasone ; liver
Abstract :	[en] We present stable transformed human mammary cell lines displaying highly inducible steroid receptor-mediated luciferase reporter gene expression. The utilization of a cell-based assay for detection of steroid agonists and antagonists down to 0.3 ng g(-1) is described and its application to the analysis of bovine samples is discussed. In particular, the use of the assays to detect illegal progesterone or androgen treatment has to take into account the presence of natural endogenous hormones. (C) 2002 Elsevier Science B.V. All rights reserved.
Permalink :	http://hdl.handle.net/2268/8708

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