ORBi: Scippo Marie-Louise - Recombinant human estrogen, androgen and progesterone receptors for detection of potential endocrine disruptors

Reference : Recombinant human estrogen, androgen and progesterone receptors for detection of potenti...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology Physical, chemical, mathematical & earth Sciences : Chemistry
	To cite this reference:	http://hdl.handle.net/2268/8706

Title :	Recombinant human estrogen, androgen and progesterone receptors for detection of potential endocrine disruptors
Language :	English
Author, co-author :	Scippo, Marie-Louise [Université de Liège - ULg > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires >]
	Argiris, Catherine [> > > >]
	Van de Weerdt, Cécile [Université de Liège - ULg > Département des sciences de la vie > Biologie et génétique moléculaire >]
	Muller, Marc [Université de Liège - ULg > Département des sciences de la vie > Biologie et génétique moléculaire >]
	Willemsen, Philippe [> > > >]
	Martial, Joseph [Université de Liège - ULg > Département des sciences de la vie > Biologie et génétique moléculaire >]
	Maghuin-Rogister, Guy [Université de Liège - ULg > Département de sciences des denrées alimentaires > Département de sciences des denrées alimentaires >]
Publication date :	2004
Journal title :	Analytical and Bioanalytical Chemistry
Publisher :	Springer-Verlag Heidelberg
Volume :	378
Issue/season :	3
Pages :	664-669
Audience :	International
ISSN :	1618-2642
City :	Heidelberg
Keywords :	[en] endocrine disruptors ; steroid receptors ; binding assay ; pesticides ; radioreceptor assay
Abstract :	[en] This work reports the binding capacity of various chemicals (so-called endocrine disruptors) to recombinant human steroid receptors (hERalpha, hPR and hAR). The tested chemicals are organochlorine insecticides (DDT and its metabolites, methoxychlor, aldrin, dieldrin, chlordecone, lindane, trichlorobenzene), estrogenic insecticides (endosulfan, toxaphene, nonachlor), herbicides (alachlor and atrazine), fungicides (benomyl and vinclozolin), industrial chemicals (nonylphenol, bisphenol A, diphenylphtalate), antioxidants (butylated hydroxyanisol) and some phytoestrogens. Except for phytoestrogens, most of the tested chemicals (DDT and its metabolites, aldrin, alpha- and beta-endosulfan, toxaphen, trans-nonachlor) show higher affinities for hPR than for hERalpha, indicating that the interaction with the progesterone receptor could contribute to the endocrine-disrupting effects imputed to these chemicals. We propose to use binding assays using recombinant human steroid receptors as screening tools for the detection of endocrine disruptors in various samples.
Permalink :	http://hdl.handle.net/2268/8706

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