Article (Scientific journals)
Enhancement of steroid receptor-mediated transcription for the development of highly responsive bioassays
Willemsen, Philippe; Scippo, Marie-Louise; Maghuin-Rogister, Guy et al.
2005In Analytical and Bioanalytical Chemistry, 382 (4), p. 894-905
Peer Reviewed verified by ORBi
 

Files


Full Text
Willemsen et al_2005.pdf
Publisher postprint (495.55 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
steroid hormones; steroid receptors; bioassay; hsp; PKA; PKC
Abstract :
[en] We have previously generated several transformed human mammary cell lines for the detection of steroid receptor-mediated activities and used these cell lines to detect and characterize steroid hormone (ant)agonistic compounds. In this report, we describe the specific optimization procedures used to enhance receptor-mediated transcription through the human glucocorticoid, progesterone and androgen receptors, respectively. Sodium arsenite-induced chemical stress leads to a substantial and specific increase in the glucocorticold receptor-mediated transcription, resulting in maximal stimulations of more than 2000-fold by the agonist dexamethasone. Similarly, a combined treatment with forskolin (an activator of adenylate cyclase) and trichostatin A (an inhibitor of histone deacetylases) leads to a synergistic enhancement of progesterone or androgen stimulation, resulting in a maximal induction of more than 200-fold or about 100-fold, respectively. The enhanced responses to specific steroids are mediated by the corresponding nuclear receptor. We show that by using these enhanced transcriptional stimulation protocols, it is possible to detect lower amounts of steroid hormones without substantially affecting the relative biological activities of various agonists. Finally, the application of these enhanced reporter cell assays to real biological samples from meat-producing animals is evaluated, and some validation parameters are presented.
Disciplines :
Biochemistry, biophysics & molecular biology
Chemistry
Author, co-author :
Willemsen, Philippe
Scippo, Marie-Louise  ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Maghuin-Rogister, Guy ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires > Département de sciences des denrées alimentaires
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Muller, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Language :
English
Title :
Enhancement of steroid receptor-mediated transcription for the development of highly responsive bioassays
Publication date :
2005
Journal title :
Analytical and Bioanalytical Chemistry
ISSN :
1618-2642
eISSN :
1618-2650
Publisher :
Springer Heidelberg, Heidelberg, Germany
Volume :
382
Issue :
4
Pages :
894-905
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 12 March 2009

Statistics


Number of views
63 (13 by ULiège)
Number of downloads
2 (2 by ULiège)

Scopus citations®
 
16
Scopus citations®
without self-citations
11
OpenCitations
 
13

Bibliography


Similar publications



Contact ORBi