Reference : Comprehensive two-dimensional gas chromatography with isotope dilution time-of-flight...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/8703
Comprehensive two-dimensional gas chromatography with isotope dilution time-of-flight mass spectrometry for the measurement of dioxins and polychlorinated biphenyls in foodstuffs - Comparison with other methods
English
Focant, Jean-François mailto [Université de Liège - ULg > Département de chimie (sciences) > Chimie analytique]
Eppe, Gauthier mailto [Université de Liège - ULg > > Centre interfac. d'analyse des résidus en traces (CART) >]
Scippo, Marie-Louise mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires >]
Massart, Anne-Cécile [Université de Liège - ULg > > Centre interfac. d'analyse des résidus en traces (CART) >]
Pirard, Catherine [> >]
Maghuin-Rogister, Guy mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Département de sciences des denrées alimentaires >]
De Pauw, Edwin mailto [Université de Liège - ULg > > Chimie physique, spectrométrie de masse >]
2005
Journal of Chromatography. A
Elsevier Science Bv
1086
1-2
45-60
Yes (verified by ORBi)
International
0021-9673
Amsterdam
[en] comprehensive two-dimensional gas chromatography (GC x GC) ; time-of-flight mass spectrometry (TOF-MS) ; quadrupole ion storage mass spectrometry (QIST-MS) ; tandem-in-time mass spectrometry (MS/MS) ; high-resolution mass spectrometry (HRMS) ; dioxin-responsive chemically activated ; luciferase gene expression (DR-CALUX) ; polychlorinated dibenzo-p-dioxins (PCDDs) ; polychlorinated dibenzofurans (PCDFs) ; polychlorinated biphenyls (PCBs)
[en] A comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC x GC-TOF-MS) experimental setup was tested for the measurement of seven 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins (PCDDs), ten 2,3,7,8-substituted polychlorinated dibenzofurans (PCDFs), four non-ortho-polychlorinated biphenyls (PCBs), eight mono-ortho-PCBs, and six indicator PCBs (Aroclor 1260) in foodstuff samples. A 40 m RTX-500 (0.18 mm I.D., 0.10 mu m df) was used as the first dimension (D-1) and a 1.5 nn BPX-50 (0.10 mm I.D., 0.10 mu m df) as the second dimension (2 D). The GC x GC chromatographic separation was completed in 45 min. Quantification was performed using C-13-label isotope dilution (11)). Isotope ratios of the selected quantification ions were checked against theoretical values prior to peak assignment and quantification. The dynamic working range spanned three orders of magnitude. The lowest detectable amount of 2,3,7,8-TCDD was 0.2 pg. Fish, pork, and milk samples were considered. On a congener basis, the GC x GC-ID-TOF-MS method was compared to the reference GC-ID high resolution mass spectrometry (HRMS) method and to the alternative GC-ID tandem-in-time quadrupole ion storage mass spectrometry (QIST-MS/MS). PCB levels ranged from low picogram (pg) to low nanogram (ng) per gram of sample and data compared very well between the different methods. For all matrices, PCDD/Fs were at a low pg level (0.05-3 pg) on a fresh weight basis. Although congener profiles were accurately described, RSDs of GC x GC-ID-TOF-MS and GC-QIST-MS/MS were much higher than for GC-ID-HRMS, especially for low level pork and milk. On a toxic equivalent (TEQ) basis, all methods, including the dioxin-responsive chemically activated luciferase gene expression (DR-CALUX) assay, produced similar responses. A cost comparison is also presented. (c) 2005 Elsevier B.V. All rights reserved.
Centre Interfacultaire d'Analyse des Résidus en Traces - CART
http://hdl.handle.net/2268/8703
10.1016/j.chroma.2005.05.090

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