Reference : Dioxin analysis in feed: cell-based assay versus mass spectrometry method
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
Engineering, computing & technology : Multidisciplinary, general & others
http://hdl.handle.net/2268/8702
Dioxin analysis in feed: cell-based assay versus mass spectrometry method
English
Scippo, Marie-Louise mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires >]
Rybertt, Soledad [> > > >]
Eppe, Gauthier mailto [Université de Liège - ULg > > Centre interfac. d'analyse des résidus en traces (CART) >]
Massart, Anne-Cécile [Université de Liège - ULg > > Centre interfac. d'analyse des résidus en traces (CART) >]
De Pauw, Edwin mailto [Université de Liège - ULg > > Chimie physique, spectrométrie de masse >]
Maghuin-Rogister, Guy mailto [Université de Liège - ULg > Département de sciences des denrées alimentaires > Département de sciences des denrées alimentaires >]
2006
Accreditation and Quality Assurance
Springer
11
1-2
38-43
Yes
International
0949-1775
New York
[en] dioxin responsive-chemically activated luciferase gene expression (DR-CALUX) ; polychlorinated dibenzo-p-dioxins (PCDDs) ; polychlorinated dibenzofurans (PCDFs) ; polychlorinated biphenyls (PCBs) ; screening ; dioxin ; feed ; food
[en] In the determination of contaminants (dioxins, polychlorinated biphenyls, polyaromatic hydrocarbons), cell-based assays are useful methods for screening purposes: they are mainly characterized by high sample throughput and lower costs than the Mass Spectrometry (MS)-based methods. Although cell-based assays can be sensitive enough for the determination of dioxins and related substances in agreement with the presently tolerable limits in food and feed (Regulation No. 2375/2001/EC and Directive 2003/57/EC respectively), their lack of specificity make their use rather questionable in control laboratories. In this paper, we present and compare results obtained from the analysis of a limited number of feed samples by both gas chromatography-high resolution mass spectrometry (GC-HRMS) and cell-based assay (DR-CALUX: dioxin responsive-chemically activated luciferase gene expression) methods. The DR-CALUX screening led to less than 10% false non-compliant and no false compliant results. In addition, there is a good correlation between GC-HRMS and DR-CALUX data. However, these preliminary results have to be confirmed on a larger number of samples to demonstrate that total toxic equivalent (TEQ), including dioxins, furans and dioxin-like polychlorobiphenyls (PCBs) can be monitored in feed and food with a cell-based assay.
http://hdl.handle.net/2268/8702

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