Reference : Prostatic androgen repressed message-1 (PARM-1) may play a role in prostatic cell imm...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Prostatic androgen repressed message-1 (PARM-1) may play a role in prostatic cell immortalisation.
Cornet, Anne [Université de Liège - ULg > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
Hanon, Emmanuel [> > > >]
Reiter, Eric R [> > > >]
Bruyninx, Marc [> > > >]
Nguyen, Viet Ha [> > > >]
Hennuy, Benoît mailto [Université de Liège - ULg > > GIGA-Management : Plate-forme transcriptomique >]
Hennen, Georges [Centre Hospitalier Universitaire de Liège - CHU > > Endocrinologie clinique >]
Closset, Jean mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Prostate (The)
Wiley Liss, Inc.
Yes (verified by ORBi)
New York
[en] Androgen-Binding Protein/pharmacology ; Androgens/pharmacology ; Animals ; Apoptosis ; Cell Survival ; Gene Expression Regulation ; Male ; Prostate/cytology/physiology ; Prostatic Neoplasms/physiopathology ; Rats ; Rats, Wistar ; Telomerase/pharmacology ; Tumor Cells, Cultured
[en] BACKGROUND: Prostatic androgen-repressed message-1 (PARM-1) has been cloned from the prostate. The transcript of the PARM-1 gene is overexpressed during regression of the prostate after androgen withdrawal. The regulation of PARM-1 by androgens is limited to this organ. We have studied the effects of PARM-1 overexpression in malignant prostate cells. METHODS: The PARM-1 cDNA was introduced into the rat cancer cell line MAT LyLu along with a doxycycline-dependent regulator. RESULTS: Maximal expression of PARM-1 (fivefold induction) was achieved by incubating the cells with 2 microM doxycycline for 48 hr. A study investigating the effect of PARM-1 overexpression on the transcription of 588 genes has shown that the TLP1 gene (encoding rat telomerase protein component 1) was the most up-regulated (fourfold). In addition, a dose-dependent increase in telomerase activity was observed in cells overexpressing PARM-1. In vivo, the androgen-deprived prostate showed an increased TLP1 level and increased telomerase activity. CONCLUSIONS: Increased telomerase activity is often associated with the immortalisation of cancer cell lines, particularly prostatic ones. This could mean that PARM-1 is involved, via increased telomerase activity, in a survival program enabling certain prostatic cells to resist apoptosis, thus conferring a selective advantage to pre-cancerous or cancerous cells.
Researchers ; Professionals
Copyright 2003 Wiley-Liss, Inc.

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