Prostatic androgen repressed message-1 (PARM-1) may play a role in prostatic cell immortalisation.

Androgen-Binding Protein/pharmacology; Androgens/pharmacology; Animals; Apoptosis; Cell Survival; Gene Expression Regulation; Male; Prostate/cytology/physiology; Prostatic Neoplasms/physiopathology; Rats; Rats, Wistar; Telomerase/pharmacology; Tumor Cells, Cultured

Abstract :

[en] BACKGROUND: Prostatic androgen-repressed message-1 (PARM-1) has been cloned from the prostate. The transcript of the PARM-1 gene is overexpressed during regression of the prostate after androgen withdrawal. The regulation of PARM-1 by androgens is limited to this organ. We have studied the effects of PARM-1 overexpression in malignant prostate cells. METHODS: The PARM-1 cDNA was introduced into the rat cancer cell line MAT LyLu along with a doxycycline-dependent regulator. RESULTS: Maximal expression of PARM-1 (fivefold induction) was achieved by incubating the cells with 2 microM doxycycline for 48 hr. A study investigating the effect of PARM-1 overexpression on the transcription of 588 genes has shown that the TLP1 gene (encoding rat telomerase protein component 1) was the most up-regulated (fourfold). In addition, a dose-dependent increase in telomerase activity was observed in cells overexpressing PARM-1. In vivo, the androgen-deprived prostate showed an increased TLP1 level and increased telomerase activity. CONCLUSIONS: Increased telomerase activity is often associated with the immortalisation of cancer cell lines, particularly prostatic ones. This could mean that PARM-1 is involved, via increased telomerase activity, in a survival program enabling certain prostatic cells to resist apoptosis, thus conferring a selective advantage to pre-cancerous or cancerous cells.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Cornet, Anne ; Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies

Hanon, Emmanuel

Reiter, Eric R

Bruyninx, Marc

Nguyen, Viet Ha

Hennuy, Benoît ; Université de Liège - ULiège > GIGA-Management : Plate-forme transcriptomique

Hennen, Georges ; Centre Hospitalier Universitaire de Liège - CHU > Endocrinologie clinique

Closset, Jean ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques

Language :

English

Title :

Prostatic androgen repressed message-1 (PARM-1) may play a role in prostatic cell immortalisation.

Publication date :

2003

Journal title :

Prostate

ISSN :

0270-4137

eISSN :

1097-0045

Publisher :

Wiley Liss, Inc., New York, United States - New York

Volume :

Issue :

Pages :

220-30

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 03 March 2011

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