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Topoisomerase I-mediated DNA relaxation as a tool to study intercalation of small molecules into supercoiled DNA.
Peixoto, Paul; Bailly, Christian; David-Cordonnier, Marie Hélène
2010In Drug-DNA Interaction Protocols
Peer reviewed
 

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Keywords :
Bacteria/cytology/genetics; Cell Death; Cesium/pharmacology; Chlorides/pharmacology; DNA Topoisomerases, Type I/metabolism; DNA, Superhelical/chemistry/genetics/isolation & purification/metabolism; Electrophoresis, Agar Gel; Ethidium/metabolism/pharmacology; Genome, Bacterial/genetics; Humans; Intercalating Agents/metabolism/pharmacology; Nucleic Acid Amplification Techniques; Plasmids/genetics/isolation & purification
Abstract :
[en] Several biochemical and biophysical methods are available to study the intercalation of a small molecule between two consecutive base pairs of DNA. Among them, the topoisomerase I-mediated DNA relaxation assay has proved highly efficient, relatively easy to handle and very informative to investigate drug binding to DNA. The test relies on the use of a supercoiled plasmid to mimic the topological constraints of genomic DNA. The three main components of the assay - the topoisomerase I enzyme, DNA helix and intercalating small molecules - are presented here in a structural context. The principle of the assay is described in detail, along with a typical experimental protocol.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Peixoto, Paul 
Bailly, Christian
David-Cordonnier, Marie Hélène
Language :
English
Title :
Topoisomerase I-mediated DNA relaxation as a tool to study intercalation of small molecules into supercoiled DNA.
Publication date :
2010
Main work title :
Drug-DNA Interaction Protocols
Publisher :
Springer, United States
Pages :
235-56
Peer reviewed :
Peer reviewed
Available on ORBi :
since 03 March 2011

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