Article (Scientific journals)
Effects of Hyperchloremia on Blood Oxygen Binding in Healthy Calves
Cambier, Carole; Detry, B.; Beerens, Dominique et al.
1998In Journal of Applied Physiology, 85 (4), p. 1267-1272
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Keywords :
red blood cells; calves; hyperchloremia
Abstract :
[en] Three different levels of hyperchloremia were induced in healthy Friesian calves to study the effects of chloride on blood oxygen transport. By infusion, the calves received either 5 ml/kg of 0.9% NaCl (low-level hyperchloremia; group A), 5 ml/kg of 7.5% NaCl (moderate hyperchloremia; group B), or 7.5 ml/kg of 7.5% NaCl (high-level hyperchloremia; group C). Blood was sampled from the jugular vein and the brachial artery. Chloride concentration, hemoglobin content, arterial and venous pH, PCO2, and PO2 were determined. At each time point (0, 15, 30, 60, and 120 min), the whole blood oxygen equilibrium curve (OEC) was measured under standard conditions. In groups B and C, hyperchloremia was accompanied by a sustained rightward shift of the OEC, as indicated by the significant increase in the standard PO2 at 50% hemoglobin saturation. Infusion of hypertonic saline also induced relative acidosis. The arterial and venous OEC were calculated, with body temperature, pH, and PCO2 values in arterial and venous blood taken into account. The degree of blood desaturation between the arterial and the venous compartments [O2 exchange fraction (OEF%)] and the amount of oxygen released at tissue level by 100 ml of bovine blood (OEF vol%) were calculated from the arterial and venous OEC combined with the PO2 and hemoglobin concentration. The chloride-induced rightward shift of the OEC was reinforced by the relative acidosis, but the altered PO2 values combined with the lower hemoglobin concentration explained the absence of any significant difference in OEF (% and vol%). We conclude that infusion of hypertonic saline induces hyperchloremia and acidemia, which can explain the OEC rightward shift observed in arterial and peripheral venous blood.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Cambier, Carole ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Département de sciences fonctionnelles
Detry, B.
Beerens, Dominique ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Département de sciences fonctionnelles
Florquin, S.;  Université de Liège - ULiège
Ansay, Michel ;  Université de Liège - ULiège > Faculté de médecine vétérinaire
Frans, A.
Clerbaux, T.
Gustin, Pascal ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Pharmacologie, pharmacothérapie et toxicologie
Language :
English
Title :
Effects of Hyperchloremia on Blood Oxygen Binding in Healthy Calves
Publication date :
1998
Journal title :
Journal of Applied Physiology
ISSN :
8750-7587
eISSN :
1522-1601
Publisher :
American Physiological Society, United States - Maryland
Volume :
85
Issue :
4
Pages :
1267-1272
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 12 March 2009

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