[en] The rabbit is an essential model in scientific research, particularly in the study of cardiovascular diseases such as hypertension and atherosclerosis and for the investigation of the osteoarticular system. Due to the fact that various factors (environmental, microbial, stress, pain.) can influence data obtained from experiments, special care must be given to housing conditions. Sensory enrichment (olfactive, auditive, visual or tactile stimuli) and physical enrichment (nutritional and social stimuli) allow rabbits to express specific behaviours and reduce stereotypies. This synthesis reminds of the importance of these factors and gives to the researcher some ways to control them. [fr] Le lapin est un modèle essentiel en recherche scientifique, en particulier dans l’étude des maladies cardiovasculaires, telles l’hypertension et l’athérosclérose, et les investigations menées sur le système ostéo-articulaire. Divers facteurs (environnement, statut microbien, stress, douleur) peuvent induire de sérieuses répercussions sur la collecte des données expérimentales. La prise en compte de ces facteurs et leur maîtrise est indispensable à l’obtention de données reproductibles. Une attention particulière doit être accordée aux conditions d’hébergement. L’enrichissement sensoriel (stimuli olfactifs, auditifs, visuels, tactiles) et l’enrichissement physique (stimuli nutritionnels et sociaux : hébergement en groupe) permettent aux animaux d’exprimer de nombreux comportements spécifiques et de réduire les stéréotypies. Cette synthèse rappelle l’importance de ces facteurs et donne quelques recommandations aux chercheurs pour mieux les contrôler
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Dewree, R.
Drion, Pierre ; Université de Liège - ULiège > Centre animalier universitaire - Animalerie centrale
Language :
French
Title :
Towards a better use of the rabbit as an experimental model: review and perspectives
Alternative titles :
[fr] Vers une meilleure gestion du lapin en tant qu’animal de laboratoire : état des lieux et perspectives
AKITA M., ISHII K., KUWAHARA M., TSUBONE H. The daily pattern of cardiovascular parameters in Kurosawa and Kusanagi-hypercholesterolemic (KHC) rabbits. Exp. Anim., 2002, 51, 353-360.
BAKER D.G. Natural pathogens of laboratory mice, rats and rabbits and their effects on research. Clin. Microbiol. Rev., 1998, 11, 231-266.
BATCHELOR G.R. Group housing on floor pens and environmental enrichment in sandy lop rabbits. Anim. Technol., 1991, 42, 109-120.
BATCHELOR G.R. The laboratory rabbit. In : Poole T. (Ed.), The Ufaw handbook on the care and management of laboratory animals. 7th edition. Iowa State Press : Ames, 1999, 395-408.
BAUMANS V., BRAIN P.F., BRUGERE H., CLAUSING P., JENESKOG T., PERRETTA G. Pain and distress in laboratory rodents and lagomorphs. Lab. Anim., 1994, 28, 97-112.
BAUMANS V. Environmental enrichment for laboratory rodents and rabbits : requirements of rodents, rabbits, and research. ILAR J., 2005, 46, 162-170.
BILKO A., ALTBACKER V. Regular handling early in nursing period eliminates fear responses toward human beings in wild and domestic rabbits. Dev. Psychobiol., 2000, 36, 78-87.
BOERS K., GRAY G., MAHMUTOVIC Z., McCORMICK S., TURCOTTE N., ZHANG Y. Comfortable quarters for rabbits in research institutions. In : Reinhardt V., Reinhardt A. (Eds.), Comfortable quarters for laboratory animals. 9th Ed. Animal Welfare Institute : Washington, 2002, 1-13.
BROCKWAY B.P., HASSLER C.R., HICKS N. Minimizing stress during physiological monitoring. In : Niemi S.M., Willson J.E. (Eds.), Refinement and reduction in animal testing. Scientists Centre for Animal Welfare : Bethesda, 1993, 56-69.
BROOKS D.L., HULS W., LEAMON C., THOMPSON J., PARKER J., TWOMEY. Cage enrichment for female New Zealand white rabbits. Lab Anim. (NY), 1993, 22, 30-38.
CLOUGH G. Environmental effects on animals used in biomedical research. Biol. Rev. Camb. Philos. Soc., 1982, 57, 487-523.
COMMISSION AU CONSEIL ET AU PARLEMENT EUROPEEN Quatrième rapport sur les statistiques concernant le nombre d'animaux utilisés à des fins expérimentales et à d'autres fins scientifiques dans les États membres de l'Union européenne, 2005, 14 p.
CONSEIL DES COMMUNAUTES EUROPEENNES Directive 86/609/CEE du Conseil du 24 novembre 1986 concernant le rapprochement des dispositions législatives, réglementaires et administratives des États membres relatives à la protection des animaux utilisés à des fins expérimentales ou à d'autres fins scientifiques. J. Off. Commun. Eur., 1986, L358, 1-28.
CONSEIL DE L'UNION EUROPEENNE Annexe A à la Convention européenne sur la protection des animaux vertébrés utilisés à des fins expérimentales ou à d'autres fins scientifiques [Strasbourg, 18.III.1986] : lignes directrices relatives à l'hébergement et aux soins des animaux. (1986) [en ligne] Adresse URL : http://conventions.coe.int/Treaty/fr/Treaties/Html/123-A.htm Consulté le 20/10/2006.
CONSEIL DE L'UNION EUROPEENNE Groupe de travail pour la préparation de la consultation multilatérale des parties à la Convention européenne sur la protection des animaux vertébré utilisés à des fins expérimentales ou à d'autres fins scientifiques (STE 123) : données statistiques sur l'utilisation des animaux de laboratoire en Belgique en 2004. [en ligne] (août 2005) Adresse URL : http://www.coe.int/t/e/legal_affairs/legal_co- operation/biological_safety,_use_of_animals/Laboratory_animals/2005/ GT%20123%20(2005)%205%20F%20Stat%202004%20BELGIQUE.pdf Consulté le 10/09/2006. COWAN D.P., BELL D.J. Leporid social behaviour and social organisation. Mamm. Rev., 1986, 16, 169-179.
COWAN D.P. Group living in the european rabbit : mutual benefit or resource localization ? J. Anim. Ecol., 1987, 56,779-795.
DEAN S.W. Environmental enrichment of laboratory animals used in regulatory toxicology studies. Lab. Anim., 1999, 33, 309-327.
EL SHEIKH A.S., CASIDA L.E. Motility and fertility of spermatozoa as affected by increased ambient temperature. J. Anim. Sci., 1955, 14, 1446-1150.
EUROPEAN FOOD SAFETY AUTHORITY The impact of the current housing and husbandry systems on the health and welfare of farmed domestic rabbits. [en ligne] (2005) Adresse URL : http://www.efsa.eu.int/etc/medialib/efsa/science/ ahaw/ahaw_opinions/1174.Par.0010.File.dat/ahaw/ahaw_rabbit_report2.pdf Consultéle 10/09/2006.
FAY R.R. Hearing in vertebrates : a psychophysics databook. Hill-Fay Associates : Winnetka, 1988, 621 p.
FLECKNELL P.A. The relief of pain in laboratory animals. Lab. Anim., 1984, 18, 147-160.
FLECKNELL P. Pain assessment, alleviation and avoidance in laboratory animals. Anzccart News, 1999, 12, 1-6.
FLECKNELL P.A. Analgesia of small mammals. Vet. Clin. North. Am-Exot. Anim. Pract., 2001, 4, 47-56.
FLECKNELL P.A., CRUZ I.J., LILES J.H., WHELAN G. Induction of anaesthesia with halothane and isoflurane in the rabbit: a comparison of the use of a face-mask or an anaesthetic chamber. Lab. Anim., 1996, 30, 67-74.
FOX R.R., MEIR.H., BEDIGIAN H.G., CRARY D.D. Genetics of transplacentally induced tetragenic and carcinogenic effect in rabbits treated with N-nitroso-Nethylurea. J. Natl. Cancer. Inst., 1982, 69, 1411-1417.
FOX R.R. Taxonomy and genetics. In : Weisbroth S.H., Flatt R.E., Kraus A.L. (Eds.), The biology of the laboratory animal medicine. Academic Press : New York, 1974, 1-22.
FOX R.R. The rabbit as a research subject. Physiologist, 1984, 27, 393-402.
FUJIWARA K. Health assessment for laboratory rodent production colonies. Lab. Anim. Sci., 1980, 30, 298-303.
GERSON P. The modification of «traditional» caging for experimental laboratory rabbits and assessment by behavioural study. Anim. Technol., 2000, 51, 13-36.
GORSKA P. Principles in laboratory animal research for experimental purposes. Med. Sci. Monit., 2000, 6, 171-180.
GUNN-DORE D. Comfortable quarters for laboratory rabbits. In : Reinhardt V. (Ed.), Comfortable quarters for laboratory animals. Animal Welfare Institute : Washington, 1997, 46-54.
GUNN D., MORTON D.B. Inventory of the behaviour of New Zealand white rabbits in laboratory cages. Appl. Anim. Behav. Sci., 1995, 45, 277-292.
HANSEN L.T., BERTHELSEN H. The effect of environmental enrichment on the behaviour of caged rabbits (Oryctolagus cuniculus). Appl. Anim. Behav. Sci., 2000, 68, 163-178.
HARRIS I. The laboratory rabbit. ANZCCART News, 1994, 4, 1-8.
HEATH M., STOTT E. Housing rabbits the unconventional way. Anim. Technol., 1990, 41, 13-25.
HEDENQVIST P., ROUGHAN J.V., ANTUNES L., ORR H., FLECKNELL P.A. Induction of anaesthesia with desflurane and isoflurane in the rabbit. Lab. Anim., 2001, 35, 172-179.
HELD S.D.E., TURNER R.J., WOOTTON R.J. The behavioural repertoire of non-breeding group-housed female laboratory rabbits (Oryctolagus cuniculus). Anim. Welf., 2001, 10, 437-443.
HELLEBREKERS L.J., De BROER E.-J.W., VAN ZUYLEN M.A., VOSMEER H.A. Comparison between medetomidine-ketamine and medetomidine-propofol anaesthesia in rabbits. Lab. Anim., 1997, 31, 58-69.
HOUDEBINE L.-M. Les animaux transgéniques permettent-ils de faire progresser la recherche médicale. [en ligne] (1998) Adresse URL : http://www.inra.fr/actualites/DOSSIERS/OGM/houd.htm Consulté le 5/09/2006.
HULS, W.L., BROOKS D.L., BEANKNUDSEN D. Response of adult New Zealand White rabbits to enrichment objects and paired housing. Lab. Anim. Sci., 1991, 41, 609-612.
INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE L'état sanitaire de l'animal de laboratoire. [en ligne] (03/05/2005) Adresse URL : http:// www.inserm.fr/fr/partenaires/qualite/recherche_pre_clinique/animal/ etatsanitaire.html. Consulté le 16/09/2006.
JACKSON G. Intestinal stasis and rupture in rabbits. Vet. Rec., 1991, 129, 287-289.
JEFFREY I.E., FOSTER P.M.D. Laboratory animal science issues in the design and conduct of studies with endocrine-active compound. ILAR J., 2004, 4, 417-424.
JILGE B. The rabbit : a diurnal or a nocturnal animal? J. Exp. Anim. Sci., 1991, 34, 170-183.
JONES S.E., PHILLIPS C.J.C. The effect of mirrors on welfare of caged rabbits. Anim. Welf., 2005, 14, 195-202.
KALAGASSY E.B. CARBONE L.G., HOUPT K.E. Effect of castration on rabbits housed as littermate pairs. J. Appl. Anim. Welf. Sci, 1999, 2, 111-121.
KPODEKON M., RIDEAUD P., COUDERT P. Pasteurelloses du lapin. Rev. Med. Vét, 1999, 150, 221-232.
KROHN T.C., RITSKES-HOITINGA J., SVENDSEN P. The effect of feeding and housing on the behaviour of the laboratory rabbit. Lab. Anim., 1999, 33, 101-107.
LEBAS F., COUDERT P., DE ROCHAMBEAU H., THEBAULT R.G. Le lapin, élevage et pathologie. Food and Agriculture Organization of the United Nations : Rome, 1996, 227 p.
LEBAS F. l'élevage du lapin en zone tropicale. Cuniculture., 2004, 31, 3-10.
LEHMANN M. Interference of restricted environment-as found in battery cages-with normal behaviour of young fattening rabbits. In : Auxilia T. (Ed.), Rabbit production systems including welfare. European Communities : Luxembourg, 1987, 257-268.
LEHMANN M. Social behaviour of young domestic rabbits under semi-natural conditions. Appl. Anim. Behav. Sci., 1991, 32, 269-292.
LIDEFORS L. Behavioural effect of environmental enrichment for individually caged rabbits. Appl. Anim. Behav. Sci., 1997, 52, 157-158.
LILES J.H., FLECKNELL P.A. The use of non-steroidal anti-inflammatory drugs for relief of pain in laboratory rodents and rabbits. Lab. Anim., 1992, 26, 241-255.
LOCKLEY R.M. Social structure and stress in the rabbit warren. J. Anim. Ecol., 1961, 30, 385-423.
LOVE J.A., HAMMOND K. Group-housing rabbits. Lab. Anim., 1991, 20, 37-43.
LOVE J.A. Group housing: meeting the physical and social needs of the laboratory rabbit. Lab. Anim. Sci., 1994, 44, 5-11.
MARR J.M., GNAM E.C., CALHOUN J., MADER J.T. A non-stressful alternative to gastric gavage for oral administration of antibiotics in rabbits. Lab. Animal., 1993, 22, 47- 49.
MIRABITO L., DUMONT F., GALLIOT P., SOUCHET C. Logement collectif des lapines reproductrices : conséquences sur le comportement. In : Institut technique de l'aviculture (Ed.), 11e Journées de la recherche Cunicole : Paris, 29-30 novembre 2005, 57-60.
MIRABITO L. Logement et bien-être du lapin : les nouveaux enjeux. In : Institut technique de l'aviculture (Ed.), 10e Journées de la recherche Cunicole : Paris, 19-20 novembre 2003, 163-172.
MORISSE J.P., BOILETOT E., MARTRENCHAR A. Grillage ou litière : choix par le lapin et incidence sur le bien-être. In : Institut technique de l'aviculture (Ed), 8e Journées de la recherche Cunicole : Paris, 9-10 juin 1999, 63-66.
MORTON D. Enrichment techniques for rodents and rabbits. In : niemi S.M., Venable J.S., Guttman H.N. (Eds.), Rodents and rabbits : current research issues. Scientists Center for Animal Welfare : Washington, 1994, 20-27.
MORTON D.B., JENNINGS M., BATCHELOR G.R., BELL D., BRIKE L., DAVIES K., EVELEIGH J.R., GUNN D., HEATH M., HOWARD B., KODER P., PHILLIPS J., POOLE T., SAINSBURY A.W., SALES G.D., SMITH D.J..A., STAUFFACHER M., TURNER R.J. Refinements in rabbit husbandry : second report of the BVAAWF/FRAME/RSPCA/UFAW Joint Working group on refinement. Lab. Anim., 1993, 27, 301-329.
MYKYTOWYCZ R. Social behaviour of an experimental colony of wild rabbit (Oryctolagus cuniculus L.) 1. Establishment of the colony. CSIRO Wildl. Res., 1958, 3, 7-25.
NAYFIELD K.C., BESCH E.L. Comparative responses of rabbits and rats to elevated noises. Lab. Anim. Sci., 1981, 31, 386-390.
NERENS R.M., LEVENSQUE M.J., CORNHILL J.F. Social environment as a factor of diet induced atherosclerosis. Science, 1980, 208, 1475-1476.
OKABE T.A., KISHIMOTO C., SHIMADA.K., MURAYAMA T., YOKODE M., KITA T. Effects of MCI-186 (edaravone), a novel free radical scavenger, upon experimental atherosclerosis in apolipoprotein E-deficient mice. Circ. J., 2006, 70, 1216-1219.
ORR H.E., ROUGHAN J.V. FLECNELL P.A. Assessment of ketamine and medetomidine anaesthesia in the domestic rabbit. Vet. Anest. Analg., 2005, 32, 271-279.
OTTESEN J.L, WEBER A, GURTLER H, MIKKELSEN L. F. New housing conditions : improving the welfare of experimental animals. Altern. Lab. Anim., 2004, 32, 397-404.
PATTON N.M. Colony husbandry. In : Manning P.J., Ringler D.H., Newcomer C.E. (Eds.), The biology of the laboratory rabbits. 2nd Ed. Academic Press : San Diego, 1994, 27-45.
PODBERSCEK A.L., BLACKSHAW J.K., BEATTIE A.W. The effects of repeated handling by familiar and unfamiliar people on rabbits in individual cages and group pens. Appl. Anim. Sci., 1991, 28, 365-373.
RAJE S., STEWART L. Group housing male New Zealand white rabbits. Lab. Anim., 1997, 28, 36-38.
RATHORE A.K. High temperature exposure of male rabbits: fertility of does mated to bucks subjected to 1 and 2 days heat treatment. Br. Vet. J., 1970, 126, 168-172.
REHBINDER C., BANEUX P., FORBES D., VAN HERCK H., NICKLAS W., RUGAYA Z., WINKLER G. FELASA recommendations for health monitoring of mouse, rat, hamster, gerbil, guinea pigs and rabbit experimental units. Lab. Anim., 1996, 30, 193-208.
REINHARDT V. Common husbandry-related variables in biomedical research with animals. Lab. Anim., 2004, 38, 213-235.
ROBERTS S.C. Group-living and consortships in two populations of European rabbits (Oryctolagus cuniculus). J. Mammal., 1987, 68, 28-38.
ROMMERS J., MEIJERHOF R. The effect of different floor types on footpad injuries of rabbit does. In : Proceedings of the 6th world rabbit congress, Toulouse, France, 9-12 July 1996, 431-436.
SATO K., CHATANI F., SATO S. Circadian and short-term variabilities in blood pressure and heart rate measured by telemetry in rabbits and rats. J. Auton. Nerv. Syst., 1995, 54, 235-246.
RUSSELL W.M.S., BURCH R.L. The principles of humane experimental technique. Methuen : London, 1959, 238 p.
SPRIET M.P., GIRARD C.A., FOSTER S.F., HARASIEWICZ K, HOLDSWORTH D.W., LAVERTY S. Validation of a 40 MHz B-scan ultrasound biomicroscope for the evaluation of osteoarthritis lesions in an animal model. Osteoarthr. Cartil., 2005, 13,171-179.
STAUFFACHER M. Group housing and enrichment for breeding fattening and laboratory rabbits. Anim. Welf., 1992, 1, 105-125.
STODART E., MYERS K. A comparison of behaviour, reproduction and mortality of wild and domestic rabbits in confined population. CSIRO Wildl. Res., 1964, 9, 144-159.
SWALLOW J., ANDERSON D., BUCKWELL A., HARRIS T., HAWKINS P., KIRKWOOD J., LOMAS M., MEACHAM S., PRESCOTT M., OWEN S, QUEST R., SUTCLIFFE R., THOMPSON K. Working group report : guidance on the transport of laboratory animals. Lab. Anim., 2005, 39, 1-39.
TOTH L., JANUARY B. Physiological stabilization of rabbits after shipping. Lab. Anim. Sci., 1990, 40, 384-387.
TURNER R.J., SELBY J.I., HELD S.D.E. , HOWELLS K. J. , EVELEIGHT J.R., WOOTTON R.J. Preferred substrates for penned laboratory rabbits. Anim. Technol., 1992, 43, 185-192.
WAGNER J.L., HACKEL D.B., SAMSELL A.G. Spontaneous death in rabbits resulting from trichobezoars. Lab. Anim. Sci., 1975, 24, 826-830.
WEIHE W.H. The effect of light on animals. In : McSheehy T. (Ed.), Control of the animal house environment. Laboratory Animal Handbooks 7. Laboratory Animals Ltd : London, 1976, 63-76.
WHARY M. , PEPER R. , BORKOWSKI G., LARENCE W., FERGUSON F. The effect of group housing on research use of laboratory rabbit. Lab. Anim., 1993, 27, 330-341.
WIXON S.K. The role of the IACUC in assessing and managing pain and distress in research animals. In : Lukas V., Podolsky M.L. (Eds), The care and feeding of an IACUC : the organization and management of an institutional animal care and use committee. CRC Press : Boca Raton, 1999, 200 p.