Article (Scientific journals)
A homologous radioimmunoassay for quantification of insulin-like growth factor-binding protein-2 in blood from cattle.
Vleurick, Lieve; Renaville, Robert; VandeHaar, M. et al.
2000In Journal of Dairy Science, 83 (3), p. 452-458
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Keywords :
Animals; Blotting, Western; Cattle/blood; Circadian Rhythm; Female; Growth Hormone/pharmacology; Insulin-Like Growth Factor Binding Protein 2/blood; Pregnancy; Radioimmunoassay/methods; Reference Values; Sensitivity and Specificity; Sheep/blood; Swine/blood
Abstract :
[en] Insulin-like growth factor-I and -II (IGF-I, IGF-II) circulate in biological fluids bound to six different IGF-binding proteins that regulate IGF bioactivity. The IGF-binding protein-2 is regulated by growth hormones, and its concentration depends on nutrition and physiological state. Specific antibodies directed against bovine IGF-binding protein-2 were produced, and IGF-binding protein-2 levels in bovine blood samples were quantified by radioimmunoassay. Parallel displacement curves showed strong cross-reactivity with bovine and ovine plasma, were low with porcine plasma, and no cross-reactivity with rat or chicken plasma. Addition of IGF-I or -II to a control pool of bovine plasma did not significantly alter control IGF-binding protein-2 values in a radioimmunoassay. Six nycthemeral periods, determined for three young bulls bled on two occasions, showed that IGF-binding protein-2 plasma levels were stable throughout the day; two or three samples were sufficient to characterize the animal. Cows treated with recombinant bovine somatotropin (bST) had significantly lower serum levels of IGF-binding protein-2 than did control cows. Furthermore, IGF-binding protein-2 levels were dramatically increased at the onset of lactation. This radioimmunoassay for bovine IGF-binding protein-2, which enables quantitative assessment of IGF-binding protein-2 concentration in cattle, confirmed that IGF-binding protein-2 concentrations are depressed by administration of bST, enhanced after calving, and showed absence of diurnal variation.
Disciplines :
Agriculture & agronomy
Veterinary medicine & animal health
Author, co-author :
Vleurick, Lieve
Renaville, Robert ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
VandeHaar, M.
Hornick, Jean-Luc  ;  Université de Liège - ULiège > Département de productions animales > Nutrition des animaux domestiques
Istasse, Louis ;  Université de Liège - ULiège > Département de productions animales > Nutrition des animaux domestiques
Parmentier, Isabelle ;  Université de Liège - ULiège > Centre interfacultaire de formation des enseignants (CIFEN)
Bertozzi, C.
Van Eenaeme, Christian ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Portetelle, Daniel 
Language :
English
Title :
A homologous radioimmunoassay for quantification of insulin-like growth factor-binding protein-2 in blood from cattle.
Publication date :
2000
Journal title :
Journal of Dairy Science
ISSN :
0022-0302
eISSN :
1525-3198
Publisher :
American Dairy Science Association, Champaign, United States - Illinois
Volume :
83
Issue :
3
Pages :
452-458
452-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 12 March 2009

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