[en] Immunohistochemistry was used to characterize the distribution of leucocyte subsets in the bronchial mucosa of 11 dogs with idiopathic eosinophilic bronchopneumopathy (EBP). Formalin-fixed tissues from all dogs were included in the study, but frozen tissue from only one dog was available. MHC class II(+) cells were found in moderate numbers in the lamina propria (LP). These cells were morphologically either dendritic-like cells or macrophages, but many macrophages did not express MHC class II. Such molecules were expressed by occasional fibroblasts. L1(+) cells, which formed a relatively small component of the LP inflammatory infiltrate, were morphologically either macrophages or polymorphonuclear cells (probably eosinophils). IgA(+) plasma cells were found in varying numbers in the LP, mostly in association with glandular tissue. IgG(+) plasma cells were less common, and IgM(+) plasma cells were present in low numbers. Many CD3(+) cells were present in the LP. In the single case from which frozen tissue was available, most of the lymphocytes were labelled with CD4 marker, while smaller numbers were CD8(+) T cells. Most of the lymphocytes in this case were positively labelled with T-cell receptor (TCR)-alphabeta marker. TCR-gammadelta(+) cells, although less common, were present in significant numbers throughout the LP. CDlc(+) dendritic cells were numerous in the epithelium and in the LP, immediately beneath the basement membrane. These findings, which were similar to those described in human asthma, are suggestive of a Th2 dominant immune response in canine EBP. As in human asthma, this provides a possible basis for new forms of treatment in canine EBP.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Peeters, Dominique ; Université de Liège - ULiège > Clinique des petits animaux
Day, M. J.
Clercx, Cécile ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux
Language :
English
Title :
Distribution of leucocyte subsets in bronchial mucosa from dogs with eosinophilic bronchopneumopathy
Publication date :
2005
Journal title :
Journal of Comparative Pathology
ISSN :
0021-9975
Publisher :
Academic Press Ltd Elsevier Science Ltd, London, United Kingdom
E.M. Banks M. Kyriakidou S. Little and A.S. Hamblin Epithelial lymphocyte and macrophage distribution in the adult and fetal equine lung Journal of Comparative Pathology 120 1999 1-13
S. Becker and E.G. Daniel Antagonistic and additive effects of IL-4 and interferon-gamma on human monocytes and macrophages: Effects on Fc receptors, HLA-D antigens, and superoxide production Cellular Immunology 129 1990 351-362
A. Bellini E. Vittori M. Marini V. Ackerman and S. Mattoli Intraepithelial dendritic cells and selective activation of Th2-like lymphocytes in patients with atopic asthma Chest 103 1993 997-1005
R.M. Bloebaum J.A. Grant and S. Sur Immunomodulation: The future of allergy and asthma treatment Current Opinion in Allergy and Clinical Immunology 4 2004 63-67
P. Brandtzaeg D.B. Jones D.J. Flavell and M.K. Fagerhol Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen Journal of Clinical Pathology 41 1988 963-970
J. Brostoff T. Hall Hypersensitivity-Type 1 in: I. Roitt J. Brostoff D. Male (Eds) Immunology 2000 Mosby Edinburgh 301-318
C. Clercx D. Peeters A.J. German Y. Khelil K. McEntee A. Vanderplasschen F. Schynts P. Hansen J. Detilleux and M.J. Day An immunologic investigation of canine eosinophilic bronchopneumopathy Journal of Veterinary Internal Medicine 16 2002 229-237
C. Clercx D. Peeters F. Snaps P. Hansen K. McEntee J. Detilleux M. Henroteaux and M.J. Day Eosinophilic bronchopneumopathy in dogs Journal of Veterinary Internal Medicine 14 2000 282-291
B.M. Corcoran K.L. Thoday J.L. Henfrey J.W. Simpson A.G. Burnie and C.T. Mooney Pulmonary infiltration with eosinophils in 14 dogs Journal of Small Animal Practice 32 1991 494-502
M.J. Day Expression of major histocompatibility complex class II molecules by dermal inflammatory cells, epidermal Langerhans cells and keratinocytes in canine dermatological disease Journal of Comparative Pathology 115 1996 317-326
J.G. Ford D. Rennick D.D. Donaldson R. Venkayya C. McArthur E. Hansell V.P. Kurup M. Warnock and G. Grunig Il-13 and IFN-gamma: Interactions in lung inflammation Journal of Immunology 167 2001 1769-1777
A.J. German P.W. Bland E.J. Hall and M.J. Day Expression of major histocompatibility complex class II antigens in the canine intestine Veterinary Immunology and Immunopathology 61 1998 171-180
A.R. Glanville H.D. Tazelaar J. Theodore E. Imoto R.V. Rouse J.C. Baldwin and E.D. Robin The distribution of MHC class I and II antigens on bronchial epithelium American Review of Respiratory Disease 139 1989 330-334
P.G. Holt Regulation of antigen-presenting cell function(s) in lung and airway tissues European Respiratory Journal 6 1993 120-129
J.C. Kips K.G. Tournoy and R.A. Pauwels New anti-asthma therapies: suppression of the effect of interleukin (IL)-4 and IL-5 European Respiratory Journal 17 2001 499-506
O.M. Kon B.S. Sihra C.H. Compton T.B. Leonard A.B. Kay and N.C. Bames Randomised, dose-ranging, placebo-controlled study of chimeric antibody to CD4 (keliximab) in chronic severe asthma Lancet 352 1998 1109-1113
R.K. Kumar J. Temelkovski H.P. McNeil and N. Hunter Airway inflammation in a murine model of chronic asthma: Evidence for a local humoral immune response Clinical and Experimental Allergy 30 2000 1486-1492
B.N. Lambrecht and H. Hammad Taking our breath away: Dendritic cells in the pathogenesis of asthma Nature Reviews Immunology 3 2003 994-1003
C. Laprise M. Laviolette M. Boutet and L.P. Boulet Asymptomatic airway hyperresponsiveness: Relationships with airway inflammation and remodelling European Respiratory Journal 14 1999 63-73
P. Lydyard C. Grossi Cells involved in the immune response in; I. Roitt J. Brostoff D. Male (Eds) Immunology 2000 Mosby Edinburgh 13-26
H.T. Maecker G. Hansen D.M. Walter R.H. DeKruyff S. Levy and D.T. Umetsu Vaccination with allergen-IL-18 fusion DNA protects against, and reverses established, airway hyperreactivity in a murine asthma model Journal of Immunology 166 2001 959-965
D. Miotto P. Christodoulopoulos R. Olivenstein R. Taha L. Cameron A. Tsicopoulos A.B. Tonnel O. Fahy J.J. Lafitte A.D. Luster B. Wallaert C.E. Mapp and Q. Hamid Expression of IFN-gamma-inducible protein; monocyte chemotactic proteins 1, 3, and 4; and eotaxin in TH1- and TH2-mediated lung diseases Journal of Allergy and Clinical Immunology 107 2001 664-670
G.M. Moller S.E. Overbeek C.G. Van Helden-Meeuwsen J.M. Van Haarst E.P. Prens P.G. Mulder D.S. Postma and H.C. Hoogsteden Increased numbers of dendritic cells in the bronchial mucosa of atopic asthmatic patients: downregulation by inhaled corticosteroids Clinical and Experimental Allergy 26 1996 517-524
C. Norris M. Mellema Eosinophilic pneumonia in: L. King (Eds) Textbook of Respiratory Disease in Dogs and Cats 2004 W.B. Saunders St Louis, MO 541-547
P.M. O'Byrne M.D. Inman and E. Adelroth Reassessing the Th2 cytokine basis of asthma Trends in Pharmacological Sciences 25 2004 244-248
T. Olivry P.F. Moore V.K. Affolter and D.K. Naydan Langerhans cell hyperplasia and IgE expression in canine atopic dermatitis Archives of Dermatological Research 288 1996 579-585
Peeters, D., Day, M. J. and Clercx, C. (2005a). An immunohistochemical study of canine nasal aspergillosis. Journal of Comparative Pathology, in press.
Peeters, D., Day, M. J., Farnir, F., Moore, P. F. and Clercx, C. (2005b). Distribution of leucocyte subsets in the canine respiratory tract. Journal of Comparative Pathology, in press.
D. Plumb Glucocorticoid agents in: D. Plumb (Eds) Veterinary Drug Handbook 2002 Pharma Vet Publishing White Bear Lake, MN 387-389
R.N. Poston P. Chanez J.Y. Lacoste T. Litchfield T.H. Lee and J. Bousquet Immunohistochemical characterization of the cellular infiltration in asthmatic bronchi American Review of Respiratory Disease 145 1992 918-921
M.M. Rajamaki A.K. Jarvinen T. Sorsa and P. Maisi Clinical findings, bronchoalveolar lavage fluid cytology and matrix metalloproteinase-2 and -9 in canine pulmonary eosinophilia Veterinary Journal 163 2002 168-181
M.M. Rajamaki A.K. Jarvinen T. Sorsa and P. Maisi Collagenolytic activity in bronchoalveolar lavage fluid in canine pulmonary eosinophilia Journal of Veterinary Internal Medicine 16 2002 658-664
D.S. Robinson A.M. Bentley A. Hartnell A.B. Kay and S.R. Durham Activated memory T helper cells in bronchoalveolar lavage fluid from patients with atopic asthma: Relation to asthma symptoms, lung function, and bronchial responsiveness Thorax 48 1993 26-32
D.S. Robinson Q. Hamid S. Ying A. Tsicopoulos J. Barkans A.M. Bentley C. Corrigan S.R. Durham and A.B. Kay Predominant Th2-like bronchoalveolar T-lymphocyte population in atopic asthma New England Journal of Medicine 326 1992 298-304
E. Salik M. Tyorkin S. Mohan I. George K. Becker E. Oei T. Kalb and K. Sperber Antigen trafficking and accessory cell function in respiratory epithelial cells American Journal of Respiratory Cell and Molecular Biology 21 1999 365-379
M.A. Schon-Hegrad J. Oliver P.G. McMenamin and P.G. Holt Studies on the density, distribution, and surface phenotype of intraepithelial class II major histocompatibility complex antigen (Ia)-bearing dendritic cells (DC) in the conducting airways Journal of Experimental Medicine 173 1991 1345-1356
C.M. Schramm L. Puddington C.A. Yiamouyiannis E.G. Lingenheld H.E. Whiteley W.W. Wolyniec T.C. Noonan and R.S. Thrall Proinflammatory roles of T-cell receptor (TCR)gammadelta and TCRalphabeta lymphocytes in a murine model of asthma American Journal of Respiratory Cell and Molecular Biology 22 2000 218-225
F. Spinozzi E. Agea O. Bistoni N. Forenza and A. Bertotto Gamma delta T cells, allergen recognition and airway inflammation Immunology Today 19 1998 22-26
L. Svensson B. Lilliehook R. Larsson and A. Bucht Gammadelta T cells contribute to the systemic immunoglobulin E response and local B-cell reactivity in allergic eosinophilic airway inflammation Immunology 108 2003 98-108
Z. Zhu R.J. Homer Z. Wang Q. Chen G.P. Geba J. Wang Y. Zhang and J.A. Elias Pulmonary expression of interleukin-13 causes inflammation, mucus hypersecretion, subepithelial fibrosis, physiologic abnormalities, and eotaxin production Journal of Clinical Investigation 103 1999 779-788
N. Zimmermann G.K. Hershey P.S. Foster and M.E. Rothenberg Chemokines in asthma: Cooperative interaction between chemokines and IL-13 Journal of Allergy and Clinical Immunology 111 2003 227-242