Article (Scientific journals)
Effect of sampling method and incubation temperature on fungal culture in canine sino-nasal aspergillosis
Billen, Frédéric; Clercx, Cécile; Le Garérrès, Alain et al.
2009In Journal of Small Animal Practice, 50 (2), p. 67-72
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Keywords :
sino-nasal aspergillosis; fungal culture; incubation temperature; dog; Aspergillus fumigatus
Abstract :
[en] OBJECTIVES: To evaluate the most appropriate sampling procedure and the effect of incubation temperature on fungal culture in the diagnosis of canine sinonasal aspergillosis (SNA). METHODS: Sixteen dogs with SNA and 20 dogs with non-fungal nasal disease entered a prospective study. Nasal secretions and mucosal biopsies were collected in all dogs. Fungal plaques were also sampled in dogs with SNA. Each specimen was taken in duplicate from each dog and incubated at room temperature and 37 degrees C. RESULTS: In dogs with SNA, nasal secretions, mucosal biopsies and fungal plaques yielded fungal growth at room temperature in one, one and seven dogs, respectively, whereas fungal growth was obtained at 37 degrees C in three, 12 and 14 dogs, respectively. No specimen collected from any dog with non-fungal nasal disease yielded fungal growth at room temperature or at 37 degrees C. CLINICAL SIGNIFICANCE: The diagnosis of canine SNA is more likely to be confirmed following culture of mucosal biopsies or fungal plaques than nasal secretions sampled blindly with swabs. Incubating cultures at 37 degrees C is more likely to provide a diagnostic outcome than when samples are cultured at room temperature. Fungal culture of nasal specimens has good specificity for the diagnosis of SNA in dogs.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Billen, Frédéric  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux
Clercx, Cécile  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux
Le Garérrès, Alain
Massart, Laurent
Mignon, Bernard 
Peeters, Dominique ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Médecine interne des animaux de compagnie
Language :
English
Title :
Effect of sampling method and incubation temperature on fungal culture in canine sino-nasal aspergillosis
Publication date :
2009
Journal title :
Journal of Small Animal Practice
ISSN :
0022-4510
eISSN :
1748-5827
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
50
Issue :
2
Pages :
67-72
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 11 March 2009

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