Article (Scientific journals)
Real-time RT-PCR quantification of mRNA encoding cytokines and chemokines in histologically normal canine nasal, bronchial and pulmonary tissue
Peeters, Dominique; Peters, I. R.; Farnir, Frédéric et al.
2005In Veterinary Immunology and Immunopathology, 104 (3-4), p. 195-204
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Keywords :
canine; chemokine; cytokine; eotaxin; real-time RT-PCR; respiratory tract
Abstract :
[en] Cytokines and chemokines are likely to be involved in the pathogenesis of inflammatory diseases of the canine respiratory tract. The roles and relative amounts of these molecules have not yet been defined in the respiratory mucosa of normal dogs or dogs with naturally acquired respiratory inflammation. In the present study, real-time reverse transcriptase polymerase chain reaction (RT-PCR) assays were employed to quantify messenger RNA (mRNA) encoding the chemokines monocyte chernotactic protein (MCP)-2, eotaxin-2 and eotaxin-3, and the cytokines interleukin-4 (IL-4), IL-5, IL-6, IL-10, IL-12p40, IL-18, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta) in normal nasal, bronchial and pulmonary tissues from puppies (n = 4) and from adult dogs (n = 7). There was no significant difference in the expression of any transcript between puppies and adult dogs at any of the anatomical sites examined. The expression of mRNA encoding eotaxin-2 and eotaxin-3 increased significantly with progression from the nasal mucosa to pulmonary parenchyma but expression of MCP-2 mRNA did not show this trend. At all levels of the respiratory mucosa, the most abundant transcripts were those encoding IL-18 and TGF-beta. Transcripts encoding IL-6, IL-10, IL-12 and TNF-alpha were approximately ten-fold less abundant, and IL-4, IL-5 and IFN-gamma were the least abundant templates. There was significantly different amount of mRNA encoding IL-5, IL- 18 and TNF-alpha between particular anatomical levels of the respiratory mucosa while the mRNA expression of the other cytokines was similar at all anatomical sites. The results of the present study will enable comparisons to be made with results obtained from similar samples obtained from dogs with nasal, bronchial or pulmonary diseases. (c) 2004 Elsevier B.V. All rights reserved.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Peeters, Dominique ;  Université de Liège - ULiège > Clinique des petits animaux
Peters, I. R.
Farnir, Frédéric  ;  Université de Liège - ULiège > Département de productions animales > Biostatistique, économie, sélection animale
Clercx, Cécile  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux
Day, M. J.
Language :
English
Title :
Real-time RT-PCR quantification of mRNA encoding cytokines and chemokines in histologically normal canine nasal, bronchial and pulmonary tissue
Publication date :
2005
Journal title :
Veterinary Immunology and Immunopathology
ISSN :
0165-2427
eISSN :
1873-2534
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
104
Issue :
3-4
Pages :
195-204
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 12 March 2009

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