Article (Scientific journals)
Quantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis
Peeters, Dominique; Peters, I. R.; Clercx, Cécile et al.
2006In Veterinary Microbiology, 114 (3-4), p. 318-326
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Keywords :
canine; cytokine; chemokine; Aspergillus; immunity
Abstract :
[en] Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We investigated the nature of the local immune response mounted in canine sino-nasal aspergillosis. Quantitative RT-PCR was carried out on RNA isolated from nasal biopsies from diseased and control dogs, using specific assays designed to amplify mRNA encoding a panel of cytokines and chemokines. Canine sino-nasal aspergillosis was associated with significantly increased expression of mRNA encoding MCP-1, -2, -3 and -4, IL-8, IL-10, EL-18 and TNF-alpha relative to controls (P < 0.01) but there was no difference between groups with respect to IL-4, IL-5, IL-6, IL-12, TGF-beta, and eotaxin-2 and -3. The up-regulation of proinflammatory cytokines and chemokines related to the influx of phagocytic cells might account for the localisation of this infection to the upper respiratory tract. The up-regulation of the expression of the immunomodulatory cytokine IL-10 in nasal tissue from affected dogs might be important in limiting the extent of local tissue destruction, but might also account for the fact that infected dogs are generally unable to clear this infection spontaneously. (c) 2005 Elsevier B.V. All rights reserved.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Peeters, Dominique ;  Université de Liège - ULiège > Clinique des petits animaux
Peters, I. R.
Clercx, Cécile  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux
Day, M. J.
Language :
English
Title :
Quantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis
Publication date :
2006
Journal title :
Veterinary Microbiology
ISSN :
0378-1135
eISSN :
1873-2542
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
114
Issue :
3-4
Pages :
318-326
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 12 March 2009

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