Reference : Bronchoalveolar lavage fluids of ventilated patients with acute lung injury activate ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/852
Bronchoalveolar lavage fluids of ventilated patients with acute lung injury activate NF-kappa B in alveolar epithelial cell line: role of reactive oxygen/nitrogen species and cytokines
English
Nys, Monique mailto [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs >]
Deby-Dupont, Ginette [> > > >]
Habraken, Yvette [Université de Liège - ULg > > Virologie - Immunologie >]
Legrand-Poels, Sylvie mailto [Université de Liège - ULg > > GIGA-R : Virologie - Immunologie >]
Ledoux, Didier mailto [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs >]
Canivet, Jean-Luc [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs >]
Damas, Pierre mailto [Université de Liège - ULg > Département des sciences cliniques > Anesthésie et réanimation]
Lamy, Maurice mailto [Université de Liège - ULg > Département des sciences cliniques > Anesthésie et réanimation]
Aug-2003
Nitric Oxide
Academic Press Inc Elsevier Science
9
1
33-43
Yes (verified by ORBi)
International
1089-8603
San Diego
[en] bronchoalveolar lavage ; nuclear factor-kappa B ; bronchopneumonia ; ARDS ; cytokines ; reactive nitrogen/oxygen species
[en] In human alveolar epithelial cell line, we investigated the binding activity of NF-kappaB induced by the bronchoalveolar lavage fluids (BALs) from ventilated patients with acute lung injury (ALI), in correlation with the concentrations of inflammatory cytokines, RNOS, and the severity of the ALI. In BALs obtained in 67 patients (16 bronchopneumonia, 14 infected ARDS, 20 ARDS, and 17 ALI patients without bronchopneumonia and no ARDS), we measured endotoxin, IL-1beta, IL-8, and nitrated proteins (NTP), the activity of mycloperoxidase, and the capacity to activate the NF-kappaB in alveolar A549 cells by electrophoretic mobility shift and supershift assays. The neutrophil counts and mean IL-1beta, IL-8, myeloperoxidase, and NTP values were increased in bronchopneumonia and infected ARDS groups compared to ARDS and ALI without bronchopneumonia and no ARDS groups (P < 0.001). The number of neutrophils was correlated to those of IL-1beta, IL-8, myeloperoxidase, NTP, and endotoxin in all groups (P < 0.0001). NF-kappaB activity was induced in alveolar like cells by BALs in all groups, was higher in bronchopneumonia and infected ARDS groups (P < 0.02), and was correlated to IL-1beta (P = 0.0002), IL-8 (P = 0.02), NTP (P = 0.014), myeloperoxidase (P = 0.016), and neutrophil counts (P = 0.003). BALs of bronchopneumonia and infected ARDS patients had increased inflammatory mediators (compared to ARDS and ALI without bronchopneumonia and no ARDS patients) that correlated to neutrophil counts and to the NF-kappaB-binding activity. These mediators and NF-kappaB activation may induce an amplification of inflammatory phenomena. By in vitro studies, we confirmed that NO-derived species (10(-6) to 10(-5) M peroxynitrite and 10(-5) M nitrites) and myeloperoxidase (at concentration equivalent to that found in BALs) can participate in the NF-kappaB activation. (C) 2003 Elsevier Inc. All rights reserved.
http://hdl.handle.net/2268/852

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