Site-directed mutagenesis of penicillin-binding protein 3 of Escherichia coli: role of Val-545

bacterial proteins; base sequence; carrier proteins/*genetics; dna, complementary; escherichia coli/*chemistry/genetics/growth & development; escherichia coli proteins; hexosyltransferases; molecular sequence data; muramoylpentapeptide carboxypeptidase/*genetics; mutagenesis, site-directed; penicillin-binding proteins; peptidoglycan glycosyltransferase; peptidyl transferases; valine/genetics

Abstract :

[en] Val545 of the Escherichia coli penicillin-binding protein 3 is essential to the acyl transfer mechanism through which the active-site serine 307 is acylated by benzylpenicillin and cephalexin and to the mechanism through which the protein allows rapidly growing cells to divide.

Research center :

CIP - Centre d'Ingénierie des Protéines - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Ayala, Juan; Universidad Autonoma - Madrid > Centro de Biologica Molecular

Goffin, Colette; Université de Liège - ULiège > Centre d'ingénierie des protéines

Nguyen-Distèche, Martine ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Ghuysen, Jean-Marie ; Université de Liège - ULiège > Centre d'Ingénierie des Protéines

Language :

English

Title :

Site-directed mutagenesis of penicillin-binding protein 3 of Escherichia coli: role of Val-545

Publication date :

15 August 1994

Journal title :

FEMS Microbiology Letters

ISSN :

0378-1097

eISSN :

1574-6968

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Volume :

121

Issue :

Pages :

251-256

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

FRSM - Fonds de la Recherche Scientifique Médicale [BE]
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]

Available on ORBi :

since 17 February 2011

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Bibliography

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Gomez, Desviat, Merchante, Ayala (1993) Involvement of the NH2- and COOH-terminal ends of PBP3 of Escherichia coli on β-lactam binding, membrane localization and function of the protein. FEMS Symposium Proceedings No. 65 , M.A. de Pedro, J.V. Höltje, W. Löffelhardt, Bacterial Growth and Lysis: Metabolism and Structure of the Bacterial Sacculus; 309-318.
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