Reference : The peptidoglycan in walls of Butyribacterium rettgeri
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/85126
The peptidoglycan in walls of Butyribacterium rettgeri
English
Guinand, Micheline [Faculté de Sciences de Lyon > > Service de Chimie Biologique > > >]
Ghuysen, Jean-Marie [Université de Liège - ULg > > Service de Bactériologie > >]
Schleifer, Karl H. [Technische Hochschule - München > Institut für Angewandte Botanik > > > >]
Kandler, Otto [Technische Hochschule - München > Institut für Angewandte Botanik > > > >]
1-Jan-1969
Biochemistry
American Chemical Society
8
1
200-207
Yes (verified by ORBi)
International
0006-2960
1520-4995
Washington
DC
[en] alanine ; amidohydrolases ; amino acid sequence ; amino acids ; cell wall ; chromatography ; chromatography ; eubacterium ; glutamates ; hexosamines ; lysine ; muramidase ; ornithine ; peptide Biosynthesis ; peptide hydrolases ; polysaccharides ; proteins ; serine ; streptomyces ; analysis ; gel ; thin layer ; bacterial
[en] The wall peptidoglycan in Butyribacterium rettgeri is composed of Nα-(L-seryl-γ-D-glutamyl)-L-ornithyl-D-alanine subunits. The peptide subunits are cross-linked by means of a D-lysine or a D-ornithine residue, extending from the α-carboxyl group of the glutamic acid of one peptide subunit, to which D-lysine is linked through its є-amino group or D-ornithine through its δ-amino group, to the carboxyl group of the C-terminal D-alanine residue of another peptide subunit. D-Lysine and D-ornithine bridges occur in the ratio of 2:1.
The Streptomyces KM endopeptidase hydrolyses the Nα-( D-alanyl)-D-lysine and Nα-(D-alanyl)-D-ornithine linkages and causes wall solubilization. The walls, as they are prepared, have a low degree of cross-linking. About 59% of the peptide subunits occur as monomers (18%), dimers (24%), and trimers (17%). Higher oligomers account for the remaining 41 %. The disaccharide units obtained after sequential treatment with Streptomyces F1 endo-N-acetylmuramidase and Streptomyces N-acetylmuramyl-L-alanine amidase are all β-1,4-N-acetylglucosaminyl-N-acetylmuramic acid.
Fonds de la Recherche Fondamentale Collective
Researchers ; Professionals
http://hdl.handle.net/2268/85126
10.1021/bi00829a029

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