Article (Scientific journals)
Chemical structure of the cell wall of Mycobacterium smegmatis. I. Isolation and partial characterization of the peptidoglycan
Petit, J. F.; Adam, A.; Wietzerbin-Falszpan, J. et al.
1969In Biochemical and Biophysical Research Communications, 35 (4), p. 478-485
Peer reviewed
 

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Keywords :
carbohydrates; cell wall; chemical phenomena; chemistry; chromatography; Chromatography, Paper; chymotrypsin; disaccharides; glucosamine; hexosamines; hydrogen-ion concentration; lipids; mycobacterium; peptides; polysaccharides; solubility; trypsin; analysis; gel; isolation & purification; bacterial
Abstract :
[en] Cell walls of were prepared by French press disruption and treatment with trypsin and chymotrypsin. Free lipids were extracted by organic solvents. Part of the bound lipids were solubilized by an alkaline treatment and most of the remaining lipids and neutral sugars by mild acid hydrolysis, leaving an insoluble residue which has the composition of a peptidoglycan. This residue was solubilized by AL1 enzyme. It was possible to isolate from the lysate a tripeptide Ala-Glu-DAP, three tetrapeptides Ala-Glu-DAP-Ala and also a tetrasaccharide and a disaccharide which give equimolar amounts of glucosamine and muramic acid after acid hydrolysis. The disaccharide differs from the typical [beta]-1,4-N-acetylglucosaminyl-N-acetylmuramic acid by its Rf values in various solvents.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Petit, J. F.;  Faculté des Sciences > Institut de Biochimie
Adam, A.;  Faculté des Sciences > Institut de Biochimie
Wietzerbin-Falszpan, J.;  Faculté des Sciences > Institut de Biochimie
Lederer, E.;  Faculté des Sciences > Institut de Biochimie
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Faculté de Médecine > Service de Bactériologie
Language :
English
Title :
Chemical structure of the cell wall of Mycobacterium smegmatis. I. Isolation and partial characterization of the peptidoglycan
Publication date :
22 May 1969
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Academic Press, San Diego, United States - California
Volume :
35
Issue :
4
Pages :
478-485
Peer reviewed :
Peer reviewed
Funders :
OMS - Organisation Mondiale de la Santé [CH]
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