Article (Scientific journals)
Hypoxia-inducible Factor-1-dependent Overexpression of Myeloid Cell Factor-1 Protects Hypoxic Cells against tert-Butyl Hydroperoxide-induced Apoptosis
Piret, Jean-Pascal; Minet, Emmanuel; Cosse, Jean-Philippe et al.
2005In Journal of Biological Chemistry, 280, p. 9336
Peer Reviewed verified by ORBi
 

Files


Full Text
Piret et al 2005.pdf
Publisher postprint (620.01 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Hypoxia; apoptosis; Mcl-1; HIF-1
Abstract :
[en] Increased levels of Mcl-1 (myeloid cell factor-1) have been reported in several cancers, suggesting an important role played by Mcl-1 in cancer cell survival. Mcl-1 is an anti-apoptotic protein shown to delay or block apoptosis. In this work, using semiquantitative immunofluorescence, real-time PCR, and RNase protection assay, an increase in Mcl-1 expression was detected in hepatoma HepG2 cells incubated under hypoxia or in the presence of cobalt chloride. Through analysis of the Mcl-1 promoter sequence, a putative HIF-1 (hypoxiainducible factor-1) binding site was identified. A Mcl-1 promoter fragment containing this hypoxia-responsive element was able to bind HIF-1 in vitro. It also induced hypoxia-dependent transcription of a luciferase reporter gene, which was suppressed by anti-HIF-1 short interfering RNA. Finally, overexpression of Mcl-1 protected HepG2 cells against apoptosis induced by tertbutyl hydroperoxide as shown by inhibition of caspase-3 activation and DNA fragmentation. All these data suggest a potential anti-apoptotic role of HIF-1 that could protect cells against apoptosis under hypoxia by overexpression of the Mcl-1 protein.
Research center :
Unité de Recherche en Biologie Cellulaire
Disciplines :
Oncology
Author, co-author :
Piret, Jean-Pascal
Minet, Emmanuel
Cosse, Jean-Philippe ;  Université de Liège - ULiège > GIGA-R : Epigénétique Cellulaire et Moléculaire
Ninane, Noelle
Debacq, Christophe
Raes, Martine
Michiels, Carine
Language :
English
Title :
Hypoxia-inducible Factor-1-dependent Overexpression of Myeloid Cell Factor-1 Protects Hypoxic Cells against tert-Butyl Hydroperoxide-induced Apoptosis
Publication date :
March 2005
Journal title :
Journal of Biological Chemistry
ISSN :
0021-9258
eISSN :
1083-351X
Publisher :
American Society for Biochemistry and Molecular Biology, Baltimore, United States - Maryland
Volume :
280
Pages :
9336
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FUNDP - Facultés Universitaires Notre-Dame de la Paix [BE]
Available on ORBi :
since 15 February 2011

Statistics


Number of views
59 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
117
Scopus citations®
without self-citations
110
OpenCitations
 
99

Bibliography


Similar publications



Contact ORBi