Reference : Role of TGF-beta1-independent changes in protein neosynthesis, p38alphaMAPK, and cdc42 i...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/8485
Role of TGF-beta1-independent changes in protein neosynthesis, p38alphaMAPK, and cdc42 in hydrogen peroxide-induced senescence-like morphogenesis.
English
Chretien, Aline [>University of Namur > > >Research Unit on Cellular Biology > > >]
Dierick, Jean-Francois [>University of Brussels, Gosselies > > >Institute of Molecular Biology and Medicine > > >]
Delaive, Edouard [>University of Southern Denmark, Odense M > > >Dpt of Biochemistry and Molecular Biology > Protein Research Group > >]
Larsen, Martin Rossel [>University of Southern Denmark, Odense M > > >Dpt of Biochemistry and Molecular Biology > Protein Research Group > > > > > >]
Dieu, Marc [>University of Namur > > >Research Unit on Cellular Biology > > > > > >]
Raes, Martine [>University of Namur > > >Research Unit on Cellular Biology > > > > > >]
Deroanne, Christophe mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs > > >]
Roepstorff, Peter [>University of Southern Denmark, Odense M > > >Dpt of Biochemistry and Molecular Biology > Protein Research Group > > > > > >]
Toussaint, Olivier [>University of Namur > > >Research Unit on Cellular Biology > > > > > >]
2008
Free Radical Biology & Medicine
Elsevier Science
44
9
1732-51
Yes (verified by ORBi)
International
0891-5849
Tarrytown
NY
[en] Calmodulin-Binding Proteins/pharmacology ; Cell Aging ; Cell Survival ; Electrophoresis, Gel, Two-Dimensional ; Fibroblasts/metabolism ; Free Radicals ; Humans ; Hydrogen Peroxide/chemistry/pharmacology ; Models, Biological ; Oxidative Stress ; Phenotype ; Phosphorylation ; Transforming Growth Factor beta1/metabolism ; cdc42 GTP-Binding Protein/metabolism ; p38 Mitogen-Activated Protein Kinases/metabolism
[en] The role of TGF-beta1 in hydrogen peroxide-induced senescence-like morphogenesis has been described. The aim of this work was to investigate whether TGF-beta1-independent changes in protein synthesis are involved in this morphogenesis and to study possible mechanisms occurring earlier than TGF-beta1 overexpression. Among the multiple TGF-beta1-independent changes in protein neosynthesis, followed or not by posttranslational modifications, identified by proteomic analysis herein, those of ezrin, L-caldesmon, and HSP27 were particularly studied. Rho-GTPase cdc42 was shown to be responsible for p38(MAPK) activation, in turn triggering phosphorylation of L-caldesmon and HSP27. Cdc42 was also shown to be mainly responsible for the increase in TGF-beta1 mRNA level observed at 24 h after treatment with H(2)O(2) and onward. This study further clarified the mechanisms of senescence-like morphogenesis in addition to the previously demonstrated role of TGF-beta1 signaling pathways.
Researchers
http://hdl.handle.net/2268/8485
10.1016/j.freeradbiomed.2008.01.026

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