Nuclear Lesions During Rat Hepatocarcinogenesis. I. Measuring the Sister-Chromatid Exchanges During Initiation, Promotion and Progression of Rat Hepatocarcinogenesis Induced with Diethylnitrosamine
Herens, Christian; Jacquemart, M.; Koulischer, Lucienet al.
1995 • In Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 329 (2), p. 153-9
[en] Cytogenetic endpoints such as sister-chromatid exchanges (SCEs), chromosomal aberrations and micronuclei (MNs) have been widely used as indicators of genetic damage. However, no systematic attempts have been made to correlate the levels of these cytogenetic endpoints with the different steps of carcinogenesis. In the present report, the induction, accumulation and persistence of SCEs and high frequency cells (HFCs) were measured in liver cells during the initiation, promotion and progression steps of rat hepatocarcinogenesis induced by diethylnitrosamine (DEN). The results indicate that lesions leading to SCEs accumulate during initiation only. When DEN administration is longer than the duration of this first step, SCE values stabilize. After stopping the carcinogenic treatment, the SCE levels decrease to control values whether or not promotion and progression occur.
Disciplines :
Genetics & genetic processes
Author, co-author :
Herens, Christian ; Centre Hospitalier Universitaire de Liège - CHU > PLAN COS
Jacquemart, M.; Centre Hospitalier Universitaire de Liège - CHU
Koulischer, Lucien ; Université de Liège - ULiège > Relations académiques et scientifiques (Médecine)
Barbason, Hervé ; Université de Liège - ULiège > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Nuclear Lesions During Rat Hepatocarcinogenesis. I. Measuring the Sister-Chromatid Exchanges During Initiation, Promotion and Progression of Rat Hepatocarcinogenesis Induced with Diethylnitrosamine
Publication date :
July 1995
Journal title :
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
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