Reference : Lipopolysaccharide-mediated interferon regulatory factor activation involves TBK1-IKK...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/84269
Lipopolysaccharide-mediated interferon regulatory factor activation involves TBK1-IKK epsilon-dependent lys(63)-linked polyubiquitination and phosphorylation of TANK/I-TRAF
English
[fr] Une stimulation des macrophages par le LPS induit une phosphorylation et une polyubiquitination de la protéine d'assemblage TANK/I-TRAF
GATOT, Jean-Stéphane mailto [Free University of Brussels > Institute of Molecular Biology and Medicine > Dept. of Applied Genetics > >]
Gioia, Romain [Université de Liège - ULg > > Chimie médicale >]
Chau, Tieu-Lan [Université de Liège - ULg > Département de pharmacie > Chimie médicale >]
Patrascu, Felicia Alina [Université de Liège - ULg > > Chimie médicale >]
Warnier, Michael [> > > >]
Close, Pierre mailto [Université de Liège - ULg > Département de pharmacie > Chimie médicale >]
Chapelle, Jean-Paul mailto [Université de Liège - ULg > Département de pharmacie > Chimie médicale >]
Muraille, Eric [> > > >]
Brown, Keith [> > > >]
Siebenlist, Ulrich [> > > >]
Piette, Jacques mailto [Université de Liège - ULg > Département des sciences de la vie > Virologie - Immunologie >]
Dejardin, Emmanuel mailto [Université de Liège - ULg > > Virologie - Immunologie >]
Chariot, Alain mailto [Université de Liège - ULg > Département de pharmacie > Chimie médicale >]
26-Oct-2007
Journal of Biological Chemistry
American Society for Biochemistry and Molecular Biology
282
43
31131-31146
Yes (verified by ORBi)
International
0021-9258
1083-351X
Baltimore
MD
[en] Type I interferon gene induction relies on IKK-related kinase TBK1 and IKK epsilon-mediated phosphorylations of IRF3/7 through the Toll-like receptor-dependent signaling pathways. The scaffold proteins that assemble these kinase complexes are poorly characterized. We show here that TANK/ITRAF is required for the TBK1- and IKK epsilon-mediated IRF3/7 phosphorylations through some Toll-like receptor-dependent pathways and is part of a TRAF3-containing complex. Moreover, TANK is dispensable for the early phase of double-stranded RNA-mediated IRF3 phosphorylation. Interestingly, TANK is heavily phosphorylated by TBK1-IKK epsilon upon lipopolysaccharide stimulation and is also subject to lipopolysaccharide- and TBK1-IKK epsilon-mediated Lys(63)-linked polyubiquitination, a mechanism that does not require TBK1-IKK epsilon kinase activity. Thus, we have identified TANK as a scaffold protein that assembles some but not all IRF3/7-phosphorylating TBK1-IKK epsilon complexes and demonstrated that these kinases possess two functions, namely the phosphorylation of both IRF3/7 and TANK as well as the recruitment of an E3 ligase for Lys63-linked polyubiquitination of their scaffold protein, TANK.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/84269
also: http://hdl.handle.net/2268/3643
10.1074/jbc.M701690200

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