Article (Scientific journals)
Immunofluorescence localization of fibronectin in chondrosarcoma cartilage matrix.
Kimata, K.; Foidart, Jean-Michel; Pennypacker, J. P. et al.
1982In Cancer Research, 42 (6), p. 2384-91
Peer Reviewed verified by ORBi
 

Files


Full Text
Kimata 1982.pdf
Publisher postprint (1.59 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] In this study, we have compared the extracellular matrix components and the in vitro adhesion characteristics of normal rat epiphysial chondrocytes with those from the Swarm rat chondrosarcoma, which has many of the biochemical characteristics of normal cartilage. With the use of immunofluorescence techniques, tissue slices and chondrocytes in culture were tested for the presence of collagen types I and II, cartilage-characteristic proteoglycan, and fibronectin. Both normal and tumor matrix contained type II collagen and cartilage proteoglycan, but only the tumor matrix contained fibronectin. In culture, tumor-derived chondrocytes continued to accumulate fibronectin in their matrix, even after deposition of type II collagen and proteoglycans, while normal chondrocytes did not. When the attachment characteristics of both types of chondrocytes were compared, tumor chondrocytes required fibronectin for attachment, while normal chondrocytes used another attachment factor that had been identified previously as chondronectin. These studies suggest that, although biochemically similar to normal chondrocytes, tumor chondrocytes are no longer able to express the regulatory mechanisms for fibronectin accumulation.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Kimata, K.
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Pennypacker, J. P.
Kleinman, H. K.
Martin, G. R.
Hewitt, A. T.
Language :
English
Title :
Immunofluorescence localization of fibronectin in chondrosarcoma cartilage matrix.
Publication date :
1982
Journal title :
Cancer Research
ISSN :
0008-5472
eISSN :
1538-7445
Publisher :
American Association for Cancer Research, Inc. (AACR), Baltimore, United States - Maryland
Volume :
42
Issue :
6
Pages :
2384-91
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 February 2011

Statistics


Number of views
47 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
10
Scopus citations®
without self-citations
8

Bibliography


Similar publications



Contact ORBi