[en] Basement membrane components and collagen biosynthesis were studied in suction blisters in human skin. The basement membrane components were characterized by immunofluorescence using specific antibodies to type IV collagen, laminin and fibronectin, and collagen biosynthesis was studied by assaying galactosylhydroxylysyl glucosylatransferase. In suction blisters, the separation of epidermis and dermis occurred above the lamina lucida, indicating that the basement membrane, composed of lamina lucida and lamina densa, forms a mechanically strong entity. During the regeneration phase of blisters, type Iv collagen and laminin were not observed in the old epidermal blister roof. This indicates that keratinocytes when separated from the underlying basement membrane or connective tissue do not synthesize laminin or type IV collagen. Galactosylhydroxylysyl glucosyltransferase activity could be demonstrated in blister fluid and was about the same as in serum when expressed on the basis of protein in fresh blisters. It increased by 2-3 fold during the repair of blisters, indicating that there was local production of this enzyme. Further studies revealed that pure epidermis contained galactosylyhdroxylysyl glucosyltransferase and hydroxyprolineand this suggests that epidermis may synthesize some collagen type which, according to these studies, is not type IV (basement memebrane) collagen.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Oikarinen, A.
Savolainen, E. R.
Tryggvason, K.
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Klistala, U.
Language :
English
Title :
Basement membrane components and galactosylhydroxylysyl glucosyltransferase in suction blisters of human skin.
Anttinen H. (1977) Collagen glucosyltransferase activity in human serum. Clinica Chimica Acta 77:323.
Ben‐Ze'ev A., Farmer S.R., Penman S. (1980) Protein synthesis requires cell‐surface contact while nuclear events respond to cell shape in anchorage‐dependent fibroblasts. Cell 21:365.
Briggaman R.A., Dallford F., Wheeler C.E. (1971) Formation and origin of basal lamina and anchoring fibrils in adult human skin. Journal of Cell Biology 51:384.
Briggaman R.A., Wheeler C.E. (1975) The epidermal‐dermal junction. Journal of investigative Dermatology 65:71.
Chen L.B., Gallimore P.K., McDougail J.K. (1976) Correlation between rumor induction and large external transformation sensitive protein on the cell surface. Proceedings of the National Academy of Sciences of the United Stales of America 73:3570.
Foidart J.M., Bere E.W., Yaar M., Rennard S.I., Gullino M., Martin G.R., Katz S.I. (1980) Distribution and immunoelectron microscopic localization of laminin, a noncollagenous basement membrane glycoprotein. Laboratory Investigation 42:336.
Hassell J.R., Robey P.O., Barrach H.J., Wilczek S., Rennard S.I., Martin G.R. (1980) Isolation of a heparan sulfate‐containing proteogiycan from basement membrane. Proceedings of the National Academy of Sciences of the United States of America 77:4494.
Hintner H., Fritsch P.O., Foidart J.M., Stingl G., Schuler G., Katz S.I. (1980) Expression of basement membrane zone antigens at the dermo‐epibolic junction in organ cultures of human skin. Journal of Investigative Dermatology 74:200.
Hunter J.A.A., McVittie E., Comaish J.S. (1974) Light and electron microscopic studies of physical injury to the skin. I. Suction. British Journal of Dermatology 90:481.
Kiistala U. (1968) Suction blister device for separation of viable epidermis from dermis. Journal of Investigative Dermatology 50:129.
Kiistla U., The Suction Blister Method for In Vivo Separation of Epidermis from Dermis in Human Skin. Academic dissertation presented in the Department of Dermatology, University Central Hospital, Helsinki, 21 May.; 1976.
Kiistala U., Mustakallio K.K. (1977) Dermo‐epidermal separation with suction. Electron microscopic and histochemical study of initial events of blistering on human skin. Journal of Investigative Dermatology 48:466.
Kivihikko K.I., Laitinkn O., Prockop D.J. (1967) Modification of a specific assay for hydroxyproline in urine. Analytical Biochemistry 19:249.
Kivirikko K.I., Myllyä R. (1979) Collagen glycosyltransferases. International Review of Connective Tisssue Research 8:23.
Kurkinen M., Vaheri A., Roberts P.J., Stenman S. (1980) Sequential appearance of fibronectin and collagen in experimental granulation tissue. Laboratory Investigation 43:37.
Laitinen O., Uitto J., Hannuksela M., Mustakallio K.M. (1966) Increased soluble collagen content of affected and normal‐looking skin in dermatomyositis, lupus erythematosus and scleroderma. Annales Medicinae Experintentalis et Biolagiae Fenniae 44:507.
Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. (1951) Protein measurement with the folin‐phenol reagent. Journal of Biological Chemistry 193:265.
Middleton M.C. (1980) Evaluation of cellular injury in skin utilizing enzyme activities in suction blister fluid. Journal of Investigative Dermatology 74:219.
Myllylä R., Risteli L., Kivirikko K.I. (1975) Assay of collagen galactosyltransferase and collagen glucosyltransferase activities and preliminary characterization of enzymic reactions with transferascs from chick embryo cartilage. European Journal of Biochemistry 54:401.
Myllyla R., Risteli L., Kivirikko K.I. (1976) Collagen glucosyltransferase. Partial purification and characterization of the enzyme from whole chick embryos and chick‐embryo cartilage. European Journal of Biochemistry 61:59.
Myllylä R., Seppä H. (1979) Studies on enzymes of collagen biosynthesis and the synthesis of hydroxyproline in macrophages and mast cells. Biochemical Journal 182:311.
Porter R.R., Reid K.B.M. (1978) The biochemistry of complement. Nature 275:699.
Stenn K.S., Maori J.A., Roll F.J. (1979) Migrating epidermis produces AB2 collagen and requires continual collagen synthesis for movement. Nature 277:229.
Timpl R., Rhode H., Robey P.G., Rennard S.I., Foidart J.M., Martin G.R (1979) Laminin – a glycoprotein from basement membranes. Journal of Biological Chemistry 254:9933.
Tuderman L., Kuutti E.R., Kivirikko K.I. (1975) Radioimmunoassay for human and chick prolyl hydroxylase. European Journal of Biochemistry 60:399.
Vaheri A., Mosher D.F. (1978) High molecular weight, cell surface‐associated glycoprotein (fibronectin) lost in malignant transformation. Biochimica et Biophysica Acta 516:1.
Vermeer B.J., Reman F.C., van Gent C.M. (1979) The determination of lipids and proteins in suction blister fluid. Journal of Investigative Dermatology 73:303.
Volden G., Thune P.O. (1979) Acid hydrolases in blister fluid. 5. Influence of ultraviolet radiation in patients with polymorphic light eruption. British Journal of Dermatology 100:277.
Vracko R. (1974) Basal lamina scaffold‐anatomy and significance for maintenance of orderly tissue structure. American Journal of Pathology 77:314.
Yaoita H., Foidart J.M., Katz S.I. (1978) Localization of the collagenous component in skin basement membrane. Journal of Investigative Dermatology 70:191.
