|Reference : Magnetic solid-phase enzyme immunoassay for the detection of anti-glomerular basement me...|
|Scientific journals : Article|
|Life sciences : Biochemistry, biophysics & molecular biology|
|Magnetic solid-phase enzyme immunoassay for the detection of anti-glomerular basement membrane antibodies.|
|Druet, E. [ > > ]|
|Mahieu, P. [ > > ]|
|Foidart, Jean-Michel [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement >]|
|Druet, P. [ > > ]|
|Journal of Immunological Methods|
|Yes (verified by ORBi)|
|[en] anti-hlomerular basement membrane antibodies ; enzyme immunoassay ; rat ; mercury|
|[en] A new enzyme immunoassay has been developed for the demonstration of antiglomerular basement membrane antibodies. Magnetically responsive polyacrylamide-agarose beads (Magnogel) activated with glutaraldehyde were used to bind sonicated insoluble rat glomerular basement membranes. Both the collagenous and the non-collagenous moieties were demonstrated to be fixed on the beads. Sera from brown Norway rats with anti-glomerular basement membrane antibodies induced by HgCl2 injections were incubated with the beads. After washing, the fixed rat IgG were revealed using alkaline phosphatase labelled Fab fragments from anti-rat IgG sheep, IgGs. Comparison with a radioimmunoassay showed that results were reliable. This enzyme immunoassay has several advantages which may render this assay of considerable clinical usefulness.|
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