Reference : Magnetic solid-phase enzyme immunoassay for the detection of anti-glomerular basement me...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Magnetic solid-phase enzyme immunoassay for the detection of anti-glomerular basement membrane antibodies.
Druet, E. [ > > ]
Mahieu, P. [ > > ]
Foidart, Jean-Michel mailto [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement >]
Druet, P. [ > > ]
Journal of Immunological Methods
Elsevier Science
Yes (verified by ORBi)
[en] anti-hlomerular basement membrane antibodies ; enzyme immunoassay ; rat ; mercury
[en] A new enzyme immunoassay has been developed for the demonstration of antiglomerular basement membrane antibodies. Magnetically responsive polyacrylamide-agarose beads (Magnogel) activated with glutaraldehyde were used to bind sonicated insoluble rat glomerular basement membranes. Both the collagenous and the non-collagenous moieties were demonstrated to be fixed on the beads. Sera from brown Norway rats with anti-glomerular basement membrane antibodies induced by HgCl2 injections were incubated with the beads. After washing, the fixed rat IgG were revealed using alkaline phosphatase labelled Fab fragments from anti-rat IgG sheep, IgGs. Comparison with a radioimmunoassay showed that results were reliable. This enzyme immunoassay has several advantages which may render this assay of considerable clinical usefulness.

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