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| Reference : Magnetic solid-phase enzyme immunoassay for the detection of anti-glomerular basement me... |
| Scientific journals : Article | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/2268/83787 | |||
| Magnetic solid-phase enzyme immunoassay for the detection of anti-glomerular basement membrane antibodies. | |
| English | |
| Druet, E. [ > > ] | |
| Mahieu, P. [ > > ] | |
Foidart, Jean-Michel  [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement >] | |
| Druet, P. [ > > ] | |
| 1982 | |
| Journal of Immunological Methods | |
| Elsevier Science | |
| 48 | |
| 2 | |
| 149-57 | |
| Yes (verified by ORBi) | |
| International | |
| 0022-1759 | |
| [en] anti-hlomerular basement membrane antibodies ; enzyme immunoassay ; rat ; mercury | |
| [en] A new enzyme immunoassay has been developed for the demonstration of antiglomerular basement membrane antibodies. Magnetically responsive polyacrylamide-agarose beads (Magnogel) activated with glutaraldehyde were used to bind sonicated insoluble rat glomerular basement membranes. Both the collagenous and the non-collagenous moieties were demonstrated to be fixed on the beads. Sera from brown Norway rats with anti-glomerular basement membrane antibodies induced by HgCl2 injections were incubated with the beads. After washing, the fixed rat IgG were revealed using alkaline phosphatase labelled Fab fragments from anti-rat IgG sheep, IgGs. Comparison with a radioimmunoassay showed that results were reliable. This enzyme immunoassay has several advantages which may render this assay of considerable clinical usefulness. | |
| http://hdl.handle.net/2268/83787 | |
| 10.1016/0022-1759(82)90189-2 |
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