Reference : Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aerugi...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/83764
Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate
English
Malissard, Martine [Université Claude Bernard - Villeurbanne (France) > > Laboratoire de Biochimie Microbienne > >]
Duez, Colette mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Guinand, Micheline [Université Claude Bernard - Villeurbanne (France) > > Laboratoire de Biochimie Microbienne > >]
Vacheron, Marie-Jeanne [Université Claude Bernard - Villeurbanne (France) > > Laboratoire de Biochimie Microbienne > >]
Michel, Georges [Université Claude Bernard - Villeurbanne (France) > > Laboratoire de Biochimie Microbienne > >]
Marty, Nicole [Université Paul Sabatier - Toulouse 3 - UPS > > Laboratoire de Microbiologie > >]
Joris, Bernard mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie et génétique bactériennes - Centre d'ingénierie des protéines >]
Thamm, Iris mailto [Université de Liège - ULg > Département des sciences de la vie > Département des sciences de la vie >]
Ghuysen, Jean-Marie [Université de Liège - ULg > > Centre d'Ingénierie des Protéines > >]
1993
FEMS Microbiology Letters
Blackwell Publishing
110
1
101-106
Yes (verified by ORBi)
International
0378-1097
1574-6968
Oxford
United Kingdom
[en] alginates/*metabolism ; amino acid sequence ; bacterial proteins/chemistry/*genetics ; base sequence ; cloning, molecular ; dna, bacterial/*analysis ; electrophoresis, polyacrylamide gel ; glucuronic acid ; hexuronic acids ; molecular sequence data ; plasmids ; polysaccharide-lyases/chemistry/*genetics ; polysaccharides, bacterial/metabolism ; pseudomonas aeruginosa/*chemistry ; recombinant fusion proteins ; sequence analysis, dna
[en] The recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originates from the marine bacterium ATCC 433367 (Brown et al., Appl. Environ. Microbiol. (1991) 57, 1870-1872). The alginate lyase produced by Escherichia coli TC4 harbouring pAL-A3 was purified to protein homogeneity and the corresponding gene sequenced, giving access to the first known primary structure of an alginate lyase. The 265-amino acid residue alginate lyase showed lytic activity on a Pseudomonas aeruginosa alginate isolated from a cystic fibrosis patient. Unexpectedly, the alginate lyase thus characterized differed from that isolated from the culture medium of the bacterium ATCC 433367 (Romeo and Preston, Biochemistry (1986) 25, 8385-8391).
Centre d'Ingénierie des Protéines - CIP
Fonds de la Recherche Scientifique Médicale - FRSM ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers ; Professionals
http://hdl.handle.net/2268/83764
10.1111/j.1574-6968.1993.tb06302.x

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