Reference : Comparison of the value of measurement of serum galactomannan and Aspergillus-specific a...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/8361
Comparison of the value of measurement of serum galactomannan and Aspergillus-specific antibodies in the diagnosis of canine sino-nasal aspergillosis.
English
Billen, Frédéric mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux >]
Peeters, Dominique mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Médecine interne des animaux de compagnie >]
Peters, I. R. [University of Bristol, School of clinical Veterinary Science > Division of Veterinary Pathology, Infection and Immunity > > >]
Helps, C. R. [University of Bristol, School of Clinical Veterinary Science > Division of Veterinary Pathology, Infection and Immunity > > >]
Huynen, Pascale mailto [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
De Mol, Patrick mailto [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
Massart, L. [Université de Liège - ULG, Faculté Vétérinaire > Département des Productions Animales > Biostatistique, Economie, Sélection animale > >]
Day, M. J. [University of bristol, School of Clinical Veterinary Science > Division of Veterinary Pathology, Infection and Immunity > > >]
Clercx, Cécile mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux >]
2009
Veterinary Microbiology
Elsevier Science
133
4
358-65
Yes (verified by ORBi)
International
0378-1135
Amsterdam
The Netherlands
[en] Dog ; Sino-nasal Aspergillosis ; Serology ; Immunodiffusion ; ELISA ; Galactomannan
[en] Serology is currently used for the diagnosis of canine sino-nasal aspergillosis (SNA). However, the accuracy of serological testing using commercially available, standardized purified antigen preparations of Aspergillus (CAPurAspAg) has only been poorly documented. The aim of the present study was to assess the diagnostic value of an agar-gel double immunodiffusion (AGDD) test and an anti-Aspergillus IgG ELISA, using CAPurAspAg and the commercially available Platelia test for the detection of serum galactomannan. Sera from 17 dogs with SNA, 18 dogs with a nasal tumour (NT), 11 dogs with lymphoplasmacytic rhinitis (LPR) and 33 control dogs were tested with the 3 methods. AGDD result was positive in 76.5% of dogs with SNA, whereas all sera from dogs with non-fungal nasal disease and control dogs were negative. A positive IgG ELISA result was obtained in 88% of dogs with SNA and in 18% of dogs with LPR. All patients with NT and control dogs had a negative IgG ELISA result. The Platelia test was positive in 24% of dogs with SNA, 11% of dogs with NT, 9% of dogs with LPR and 24% of control dogs. The results of this study suggest that (1) the detection of serum Aspergillus-specific antibodies with AGDD or ELISA, using CAPurAspAg, provides excellent specificity and good sensitivity, (2) the specificity is higher for AGDD (100%) than for ELISA (96.8%) while sensitivity is higher for ELISA (88.2%) than for AGDD (76.5%) and (3) serum galactomannan quantification with the Plateliat test is unreliable for the diagnosis of canine SNA.
fondation Huynen
http://hdl.handle.net/2268/8361
10.1016/j.vetmic.2008.07.018

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
vet microb sero SNA.pdfPublisher postprint210.35 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.