Reference : Peptide inhibitors of Streptomyces DD-carboxypeptidases
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/83539
Peptide inhibitors of Streptomyces DD-carboxypeptidases
English
Nieto, Manuel [National Institute for Medical Research > > > > >]
Perkins, Harnold R. [National Institute for Medical Research > > > > >]
Leyh-Bouille, Mélina [Université de Liège - ULg > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
Frère, Jean-Marie mailto [Université de Liège - ULg > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
Ghuysen, Jean-Marie [Université de Liège - ULg > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
1-Jan-1973
Biochemical Journal
Portland Press
131
1
163-171
Yes (verified by ORBi)
International
0264-6021
1470-8728
London
United Kingdom
[en] carbon isotopes ; carboxypeptidases/antagonists & inhibitors ; chromatography ; chromatography paper ; chromatography thin layer ; electrophoresis paper ; kinetics ; oligopeptides chemical synthesis/pharmacology ; optical rotation ; streptomyces/drug effects/enzymology ; ion exchange
[en] 1. Peptides that inhibit the dd-carboxypeptidases from Streptomyces strains albus G and R61 were synthesized. They are close analogues of the substrates of these enzymes. The enzymes from albus G and R61 strains are in general inhibited by the same peptides, but the enzyme from strain R39 differs considerably. 2. The two C-terminal residues of the peptide substrates and inhibitors appear to be mainly responsible for the initial binding of the substrate to the enzymes from albus G and R61 strains. The side chain in the third residue from the C-terminus seems critical in inducing catalytic activity. 3. Experimental evidence is presented suggesting that the amide bond linking the two C-terminal residues has a cis configuration when bound to the enzymes from strains albus G and R61. 4. The peptide inhibitors are not antibiotics against the same micro-organisms.
Fonds de la Recherche Fondamentale Collective
Researchers ; Professionals
http://hdl.handle.net/2268/83539

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