Reference : Exocellular beta-lactamases of Streptomyces albus G and strains R39 and K11
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/83538
Exocellular beta-lactamases of Streptomyces albus G and strains R39 and K11
English
Johnson, Kenneth [National Research Concil of Canada > > > > >]
Dusart, Jean [Université de Liège - ULg > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
Campbell, James N. [National Research Council of Canada > Department of Microbiology > Division of Biological Sciences > >]
Ghuysen, Jean-Marie [Université de Liège - ULg > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
1-Feb-1973
Antimicrobial Agents and Chemotherapy
American Society for Microbiology (ASM)
3
2
289-298
Yes (verified by ORBi)
International
0066-4804
1098-6596
Washington
DC
[en] carboxypeptidases pharmacology ; electrophoresis polyacrylamide gel ; microbial sensitivity tests ; penicillin g ; penicillin resistance ; penicillinase ; streptomyces ; pharmacology ; isolation & purification ; drug effects ; enzymology
[en] The beta-lactamases excreted by the highly benzylpenicillin-susceptible Streptomyces strain R39 and the highly benzylpenicillin-resistant Streptomyces albus G were isolated and purified. Neither beta-lactamase exhibited dd-carboxypeptidase activity. Both were anionic at pH 8.3, did not require metal ions, and were not sensitive to iodine, but were inhibited by Cu(2+) and readily inactivated by heat. p-Chloromercuribenzoate, iodoacetate, p-aminobenzoate, and substrates and inhibitors of dd-carboxypeptidase had no effect on beta-lactamase activity. The K(m) and V(max) values for beta-lactamase activity were studied with 6-aminopenicillanic acid and with various penicillins and cephalosporins. The beta-lactamase from the related strain K11 of Streptomyces, which is intermediate in its susceptibility to benzylpenicillin, was partially purified, and its activity was compared on the various substrates.
Fonds de la Recherche Fondamentale Collective
Researchers ; Professionals
http://hdl.handle.net/2268/83538

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