Reference : Adherence to various host cell lines of Mycoplasma bovis strains differing in pathoge...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Life sciences : Microbiology
http://hdl.handle.net/2268/8345
Adherence to various host cell lines of Mycoplasma bovis strains differing in pathogenic and cultural features
English
Thomas, Anne [> > > >]
Sachse, Konrad [> > > >]
Dizier, Isabelle [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes >]
Grajetzki, C. [> > > >]
Farnir, Frédéric mailto [Université de Liège - ULg > Département de productions animales > Biostatistique, économie, sélection animale >]
Mainil, Jacques mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes >]
Linden, Annick mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Santé et pathologies de la faune sauvage >]
2003
Veterinary Microbiology
Elsevier Science Bv
91
2-3
101-113
Yes (verified by ORBi)
International
0378-1135
Amsterdam
The Netherlands
[en] Mycoplasma bovis ; cytadherence ; cell lines ; pathogenicity ; in vitro passage ; immunoblotting ; variable surface proteins
[en] Mycoplasma bovis is known to be responsible for pneumonia and arthritis in calves, as well as mastitis in dairy cows. Despite clear evidence of its pathogenic potential, little is known about mechanisms of cytadherence and the molecular factors involved. The purpose of this work was to compare adherence rates of M. bovis field strains to different host cell lines and study the effects of cloning and sub-culturing M. bovis strains on their adherence properties. Eighteen metabolically labeled M. bovis strains isolated from different pathological backgrounds were examined in adherence trials using four different host cell lines, i.e. embryonic bovine lung (EBL), embryonic bovine trachea (EBTr), Madin Darby bovine kidney (MDBK) and rabbit kidney (RK) cells. Although large interstrain variations in adherence rates (3.4-19.1%) were measured they could not be correlated to the pathological background (pneumonia, arthritis or mastitis). Adherence rates to the fibroblast cell line (EBTr) were significantly lower than those to the three epithelial cell lines (EBL, MDBK and RK). The only non-pathogenic strain (221/89) exhibited lower adherence rates than three isolates from clinical mastitis. Interestingly, adherence rates were significantly reduced after in vitro passaging. In contrast, no effect of single cloning of strains on adherence was observed. There was no general correlation between expression of variable surface proteins (Vsps) as monitored by immunoblotting and adherence rates, although alterations in Vsp expression profiles were seen as a consequence of passaging. As there is probably a large number of adhesins, variable and non-variable, on the surface of M. bovis cells the issue is very complex, and the most active components have yet to be identified.
Researchers
http://hdl.handle.net/2268/8345
also: http://hdl.handle.net/2268/135718

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
2003-Adherence to various host cell lines of M bovis strains.pdfNo commentaryPublisher postprint169.25 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.