No full text
Article (Scientific journals)
Fibronectin presence in native collagen fibrils of human fibroblasts: immunoperoxidase and immunoferritin localization.
Furcht, L. T.; Smith, D.; Wendelschafer-Crabb, G. et al.
1980In Journal of Histochemistry and Cytochemistry, 28 (12), p. 1319-33
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Abstract :
[en] Fibronectin is a major constituent of the fibroblast extracellular matrix. Fibronectin binds to collagen, mediates fibroblast adhesion to collagen, and is synthesized and secreted into the medium of cultured fibroblasts. Affinity-purified antibodies to fibronectin and collagen were localized using the peroxidase-antiperoxidase method or with ferritin-coupled secondary antibodies. Using human fibroblasts cultured under routine conditions, fibronectin and procollagen I react in a nonperiodic manner with: 1) approximately 10 nm extracellular fibrils, 2) cell membrane, and 3) membrane-associated vesicles. All fibrils react with both antibodies, suggesting some form of codistribution of fibronectin and collagen in these fibrils. Treatment with ascorbate leads to the development of a larger diameter extracellular fibril, approximately 40 nm in diameter. These large diameter fibrils are clearly collagen fibrils as documented by the procollagen antibody reaction. Importantly, fibronectin is bound to or a constituent of these "native" or cellular made collagen fibrils. Fibronectin and procollagen antibodies localized with the peroxidase-antiperoxidase method have a 70 nm axial repeat of reaction product on ascorbate-treated fibroblasts. Localization of antibodies with ferritin-labeled secondary antibodies is less satisfactory, but supports the basic observations made with the unlabeled antibody enzyme method. This observation rules out any potential criticisms. Although it is more difficult to observe with immunoferritin, there is an indication that antibodies to fibronectin react with an axial periodicity on cellular produced collagen fibrils.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Furcht, L. T.
Smith, D.
Wendelschafer-Crabb, G.
Mosher, D. F.
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Language :
English
Title :
Fibronectin presence in native collagen fibrils of human fibroblasts: immunoperoxidase and immunoferritin localization.
Publication date :
1980
Journal title :
Journal of Histochemistry and Cytochemistry
ISSN :
0022-1554
eISSN :
1551-5044
Publisher :
Histochemical Society, New York, United States - New York
Volume :
28
Issue :
12
Pages :
1319-33
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 01 February 2011

Statistics


Number of views
38 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
57
Scopus citations®
without self-citations
44
OpenCitations
 
68

Bibliography


Similar publications



Contact ORBi