Molecular weight, amino acid composition and physicochemical properties of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39

Frère, Jean-Marie; Moreno, Ramon; Ghuysen, Jean-Marie

doi:10.1042/bj1430233

Article (Scientific journals)

Molecular weight, amino acid composition and physicochemical properties of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39

Frère, Jean-Marie; Moreno, Ramon; Ghuysen, Jean-Marie

1974 • In Biochemical Journal, 143 (1), p. 233-240

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/83088

DOI
10.1042/bj1430233

PubMed
4464852

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Keywords :

amino acids/analysis; carboxypeptidases/isolation & purification; cells, cultured; centrifugation; chemistry, physical; chromatography, gel; electrophoresis, polyacrylamide gel; fydrolysis; isoelectric focusing; methods; molecular weight; muramoylpentapeptide carboxypeptidase/analysis; physicochemical phenomena; protein conformation; proteins/analysis; sodium dodecyl sulfate; spectrometry, fluorescence; streptomyces/*enzymology

Abstract :

[en] The exocellular dd-carboxypeptidase-transpeptidase from Streptomyces R39 was purified to protein homogeneity and in milligram amounts. The isolated enzyme consisted of one polypeptide chain of molecular weight about 53300. Its amino acid composition and several physicochemical properties were determined and compared with those of the exo-cellular dd-carboxypeptidase-transpeptidase from Streptomyces R61.

Disciplines :

Biochemistry, biophysics & molecular biology
Microbiology

Author, co-author :

Frère, Jean-Marie ; Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie

Moreno, Ramon; Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie

Ghuysen, Jean-Marie ; Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie

Language :

English

Title :

Molecular weight, amino acid composition and physicochemical properties of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39

Publication date :

01 October 1974

Journal title :

Biochemical Journal

ISSN :

0264-6021

eISSN :

1470-8728

Publisher :

Portland Press, London, United Kingdom

Volume :

143

Issue :

Pages :

233-240

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
FRFC - Fonds de la Recherche Fondamentale Collective [BE]

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since 28 January 2011

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