Article (Scientific journals)
Interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61, substrate and beta-lactam antibiotics. A choice of models
Frère, Jean-Marie; Ghuysen, Jean-Marie; Perkins, Harold R
1975In European Journal of Biochemistry, 57 (2), p. 353-359
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Keywords :
acyltransferases/*metabolism; bnding sites; binding, competitive; carbenicillin/metabolism; carboxypeptidases/*metabolism; kinetics; mathematics; models, chemical; penicillin g/metabolism; penicillin v/metabolism; penicillins/*metabolism; peptidyl transferases/*metabolism; protein binding; streptomyces/*enzymology
Abstract :
[en] The interaction between the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61 and beta-lactam antibiotics is a multistep process during which a rather stable enzyme - antibiotic complex is formed. This mechanism of interaction is compatible with Lineweaver-Burk plots that are typical of a competitive inhibition of the hydrolysis of the peptide donor by the antibiotic. In fact, however, the same Lineweaver-Burk plots can be obtained on the basis of a non-competitive type of inhibition. At present, a choice between the two models cannot be made.
Disciplines :
Biochemistry, biophysics & molecular biology
Microbiology
Author, co-author :
Frère, Jean-Marie ;  Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie
Perkins, Harold R;  University of Liverpool > Department of Microbiology
Language :
English
Title :
Interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61, substrate and beta-lactam antibiotics. A choice of models
Publication date :
15 September 1975
Journal title :
European Journal of Biochemistry
ISSN :
0014-2956
eISSN :
1432-1033
Publisher :
Blackwell Science, Oxford, United Kingdom
Volume :
57
Issue :
2
Pages :
353-359
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
FRFC - Fonds de la Recherche Fondamentale Collective [BE]
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